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Showing: 2641 - 2660 of 15565 results

  2. pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)

    Plasmid
    #126755
    Purpose
    Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126755-plasmid-map-sequence-id-410971

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  3. pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82704
    Purpose
    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO-L Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82704-plasmid-map-sequence-id-158051

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  4. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid
    #218710
    Purpose
    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218710-plasmid-map-sequence-id-433357

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  5. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid
    #166693
    Purpose
    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.
    Depositor
    Elvir Becirovic
    Article
    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi
    Inserts
    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE and U6
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    166693-plasmid-map-sequence-id-331043

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  6. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60226
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60226-plasmid-map-sequence-id-176980

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  10. pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82705
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    82705-plasmid-map-sequence-id-170450

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  13. hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

    Plasmid
    #154430
    Purpose
    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    Depositor
    David Liu
    Article
    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:
    Insert
    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    VRQR point mutations in SpCas9
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    154430-plasmid-map-sequence-id-304586

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  14. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60225
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    60225-plasmid-map-sequence-id-96391

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  15. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82702
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82702-plasmid-map-sequence-id-163793

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  16. pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82701
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82701-plasmid-map-sequence-id-157303

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  17. pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82703
    Purpose
    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO-L gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82703-plasmid-map-sequence-id-158053

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Showing: 2641 - 2660 of 15565 results