Skip to main content
Addgene
Showing: 2641 - 2660 of 13332 results

  13. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid
    #179527
    Purpose
    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 K233A/K234A/K253A/K263A
    Use
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed lysines 233, 234, 253, and 263 to alanine
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179527-plasmid-map-sequence-id-351733

    Enlarge

  14. pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

    Plasmid
    #166133
    Purpose
    This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance
    Depositor
    Raphael Gaudin
    Article
    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep
    Insert
    hU6-gRNA and hH1-gRNA targeting cldn1 gene
    Use
    CRISPR and Lentiviral
    Tags
    iRFP670
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166133-plasmid-map-sequence-id-333282

    Enlarge

  16. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

    Plasmid
    #135669
    Purpose
    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    sgPten
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135669-plasmid-map-sequence-id-325841

    Enlarge

  17. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

    Enlarge

  18. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

    Enlarge

Showing: 2641 - 2660 of 13332 results