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Plasmid#74602Purposeexpresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherryDepositorInsertfull length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianMutationPromoterSFFVAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX Mist-SH2 RK
Plasmid#46455DepositorInsertMist RK (Clnk Mouse)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 300-435PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin-V159N
Plasmid#118383PurposeGateway compatible donor vector with actin-V159N.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationV159NPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin-G168D,Y169D
Plasmid#118384PurposeGateway compatible donor vector with actin-G168D,Y169D.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationG168D,Y169DPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
NMRK2 gRNA (BRDN0001145387)
Plasmid#77130Purpose3rd generation lentiviral gRNA plasmid targeting human NMRK2DepositorInsertNMRK2 (NMRK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX Mist-SH2
Plasmid#46454DepositorInsertMist (Clnk Mouse)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 300-435PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
Hook3(1-522)-GFP-Psc-StrepII
Plasmid#111861PurposeC-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.DepositorInsertHook3(1-552)-GFP (HOOK3 Human)
UseTagsGFP, Psc tag, and Strep tagExpressionInsectMutationOptimised for expression in SF9 cellsPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human LIC1 G domain
Plasmid#74598Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389DepositorInserthuman dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
UseTagsN-terminal GST and Strep IIExpressionBacterialMutationPromoterT7Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TTK gRNA (BRDN0001145152)
Plasmid#75579Purpose3rd generation lentiviral gRNA plasmid targeting human TTKDepositorInsertTTK (TTK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TTK gRNA (BRDN0001146504)
Plasmid#75580Purpose3rd generation lentiviral gRNA plasmid targeting human TTKDepositorInsertTTK (TTK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TTK gRNA (BRDN0001147779)
Plasmid#75581Purpose3rd generation lentiviral gRNA plasmid targeting human TTKDepositorInsertTTK (TTK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-PODXL FBM*/PBM*
Plasmid#195221PurposeMammalian expression vector containing GFP tagged Podocalyxin. Dual mutant for HRS>AAA / lacking DTHL.DepositorInsertPODXL (PODXL Rabbit)
UseTagsExpressionMammalianMutationDual mutant for HRS>AAA / lacking DTHLPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-mRuby3-CERT(PHD)
Plasmid#221762PurposeStable expression of mRuby3-CERT(PHD) in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationamino acids 1–116PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP2A-NS3-blue
Plasmid#223532PurposeMammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.DepositorInsertCHMP2A (CHMP2A Human)
UseTagsNS3 Hepatitis C protease and mTurquoiseExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP2A-NS3-Green
Plasmid#223440PurposeMammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.DepositorInsertCHMP2A (CHMP2A Human)
UseTagsNS3 Hepatitis C protease and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-CERT(PHD)
Plasmid#221746PurposeStable expression of GFP-CERT(PHD)in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–116PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR77-DuET
Plasmid#213291PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR77 (C5AR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
F2RL1-DuET
Plasmid#213235PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertF2RL1 (F2RL1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-GST-TEV-mCherry-NAP1 (WT)
Plasmid#198036PurposeUsed for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)DepositorInsertNAP1 (AZI2 Human)
UseTagsGST-TEV and mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Creb5(T59/T61A)(HA)
Plasmid#195025PurposeGateway vector containing HA-tagged Creb5 with T59/T61A mutationDepositorInsertHA-tagged Creb5 (full length, 508aa; with with T59/T61A mutation) (CREB5 Bovine)
UseGateway cloningTags3xHAExpressionMutationT59/T61APromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only