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Plasmid#22416DepositorInsertvegf (vegfaa Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGCS2
Plasmid#85731PurposeGateway destination vectorDepositorTypeEmpty backboneUseMammalian/avian/xenopus/zebrafishTagsExpressionMutationPromoterAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10
Plasmid#199487PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10
Plasmid#199488PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10
Plasmid#199489PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10
Plasmid#199497PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
recoverin1b
Plasmid#83812Purposeexpress Zebrafish recoverin1bDepositorInsertrecoverin1b (rcvrnb Zebrafish)
UseTagsExpressionBacterialMutation*(see below)PromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
His6-Halo-TEV-Akt2
Plasmid#87606Purposeexpresses His-halo tagged at the N-terminus of human Akt2 proteinDepositorInsertAKT 2 (AKT2 Human)
UseZebrafish (danio rerio) expressionTagsHis-Halo-TevExpressionMammalianMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
recoverin2b
Plasmid#83815Purposeexpress zebrafish recoverin2bDepositorInsertrecoverin2b (rcvrn3 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ABCB1a
Plasmid#38036DepositorInsertABCB1a
UseSea urchin, zebrafish, xenopusTagsExpressionMutationPromoterSP6Available sinceJan. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2+CeGFP-ABCC9a
Plasmid#35581DepositorInsertABCC9a
UseSea urchin, zebrafish, xenopusTagseGFPExpressionMammalianMutationPromoterSP6Available sinceJan. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)
Plasmid#156415Purposekdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseZebrafish transgenesisTagsmTagBFP2ExpressionMutationmutant KRAS4A-G12DPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)
Plasmid#156416Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseZebrafish transgenesisTagsmScarlet-IExpressionMutationmutant KRAS4A-G12VPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)
Plasmid#180468Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorInsertKRAS4A (KRAS Human)
UseZebrafish transgenesisTagsV5-mScarlet-IExpressionMutationPromoterkdrlAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)
Plasmid#156414Purposekdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseZebrafish transgenesisTagsEGFPExpressionMutationmutant KRAS4B-G12VPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRIMPkit
Plasmid Kit#1000000225PurposeThe CRISPR/Cas9 Insertional Mutagenesis Protocol (CRIMP) and toolkit (CRIMPkit) is a universal and highly efficient targeted insertional mutagenesis system designed for use in zebrafish.DepositorAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-eGFP
Plasmid#221710PurposeExpression of eGFP in zebrafish embryos.DepositorInserteGFP
UseFish expressionTagsExpressionMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:redCas9; control_gRNA
Plasmid#199334PurposepDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNADepositorInsertszCREST3
Cas9
control sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2_claudink:Cas9green; control_gRNA
Plasmid#199336PurposepDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNADepositorInsertsclaudink
Cas9
control sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Membrane EGFP
Plasmid#198056PurposePlasma membrane localizing EGFP using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane EGFP (LCK Synthetic, A. victoria, Human)
UseSea urchin, mammal, zebrafishTagsEGFPExpressionMutationTandem repeat of N-terminal LCK membrane targetin…Promotersp6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only