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Plasmid#21657DepositorInsertCyclin D2 (CCND2 Human)
UseRetroviralTagsExpressionMammalianMutationmutated miR-15/16 and let-7 binding sitesPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRSV-p65T305A,S276A
Plasmid#106456PurposeNF-kB p65, kinase sites 276,305 mutatedDepositorInsertNF-kB p65, kinase sites 276,305 mutated (RELA Human)
UseTagsExpressionMammalianMutationkinase sites inactivatedPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-eGFP-Esp3I
Plasmid#81226PurposeCloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sitesDepositorInsertEsp31-Neo-Esp31 deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ
Plasmid#51495PurposepLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmidDepositorInsertsZif268:FokI
3kb filler sequence
UsePlant t-dna plasmidTagsSV40 NLSExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_Ch10
Plasmid#81224PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in PCDH15 locus on chromosome 10DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
RcSxph
Plasmid#194018PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
UseTagsHis10 and mEGFP,ExpressionMutationPromoterPolyhedrinAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE3 CTFdeltaTA-FLAG
Plasmid#217724PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE3 (ADGRE3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-344PromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
LEGO-pDHS-ITDE-AP1x6-DUSP5
Plasmid#217419PurposeLuciferase expression vector with an oncogene-inducible enhancer, 6 AP-1 sites, the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6-ITDeDepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterHuman DUSP5 promoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF2 CTF-FLAG
Plasmid#217695PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF2 (ADGRF2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-429PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE3 CTF-FLAG
Plasmid#217691PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE3 (ADGRE3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-338PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE2 CTF-FLAG
Plasmid#217690PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE2 (ADGRE2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-517PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE1 CTFdeltaTA-FLAG
Plasmid#217722PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE1 (EMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-590PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE4 CTF-FLAG
Plasmid#217692PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE4 (ADGRE4P Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-173PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF2 CTFdeltaTA-FLAG
Plasmid#217728PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF2 (ADGRF2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-435PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA1-FLAG
Plasmid#217678PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRA1 (ADGRA1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE2 CTFdeltaTA-FLAG
Plasmid#217723PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE2 (ADGRE2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-523PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSGKP_AcsgRNA
Plasmid#203807PurposepSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.DepositorInsertAcgRNA
UseCRISPRTagsExpressionMutationPromoterJ23119Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
BcrMT1B Blue Crab Metallothionein 1B
Plasmid#200290PurposeBacterial expression plasmids for production of recombinant Blue Crab Callinectes sapidus Metallothionein 1BDepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato. PGK-puro
Plasmid#136264PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomatoExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMiR-HPV16-E7/E1-miR-375-m1-5
Plasmid#53704PurposeLuciferase reporter assay for HPV16 E7/E1 with point mutation on miR-375 binding siteDepositorInsertHPV16-E7/E1
UseLuciferaseTagsExpressionMutationMutation in miR-375 binding sites m1-5 (refer to …PromoterAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only