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Plasmid#16265DepositorInsertBeta 4 Integrin (ITGB4 Human)
UseTagsExpressionMammalianMutationlacked the 70- and 7-amino acid splice variants a…PromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pLV-NT-3/D15A
Plasmid#73035PurposeExpress an NT-3-BDNF hybrid neurotrophic factor in mammalian cellsDepositorInsertNT-3/D15A (NTF3 Human)
UseLentiviralTagsExpressionMammalianMutationMutated Aspartic acid in position 15 of the matur…PromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYLL204
Plasmid#53455PurposeExpresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)InsertHA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)
UseTagsHAExpressionMammalianMutationY814FPromoterCMVAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MEKK4alpha KD
Plasmid#12188DepositorInsertMEKK4 (Map3k4 Mouse)
UseTagsHAExpressionMammalianMutationKinase dead: K1361MPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a TM delta CT
Plasmid#1797DepositorInsertFOXO3a (FOXO3 Human)
UseTagsHAExpressionMammalianMutationT32A, S253A, S315A, delta CT (aa 468-673)PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
DDX5 D248N V5
Plasmid#199577PurposeExpresses helicase dead DDX5 cloned into pLenti puroDepositorInsertDDX5 (DDX5 Human)
UseTagsV5ExpressionMammalianMutationD248NPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
myc-hIFITM3-S61A,N64A,T65A
Plasmid#104362PurposeExpresses human IFITM3-S61A,N64A,T65A with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsmycExpressionMammalianMutationmutated the following amino acids: S61A,N64A,T65APromoterCMVAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKM60
Plasmid#154159PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation4 wt NF-κB sites were replaced with Rel1A-specifi…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos3.2 (mEosFP3.2-A69T)
Plasmid#99224PurposeBacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).DepositorInsertpr-mEos3.2
UseTagsExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo-TGmiR-Luc
Plasmid#25745PurposeControl lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.DepositorInsertLuciferase miR-shRNA
UseLentiviral and RNAiTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-StefinA
Plasmid#182403PurposeBacterial expression of Vesicle Nucleating peptide-stefinA fusionDepositorInsertVNp-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Lenti-SLC35B2-sgRNA
Plasmid#154860PurposeLentiviral expression of Cas9 and sgRNA targeting SLC35B2DepositorInsertSLC35B2 sgRNA (SLC35B2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (Frag B)
Plasmid#16606DepositorInsertc-myc promoter (Frag B) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationFragB (KpnI->AccI). The KpnI site is at -1194 …PromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (Frag C)
Plasmid#16607DepositorInsertc-myc promoter (Frag C) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationFragC (AccI->PvuII). The AccI site is at -741 …PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pmiR-report-hEZH2
Plasmid#112543Purpose3'UTR reporter assayDepositorInsertEZH2 (EZH2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a T32A DBM (H212R)
Plasmid#1793DepositorInsertFOXO3a (FOXO3 Human)
UseTagsHA (YDVPDYASL)ExpressionMammalianMutationT32A, S253A, S315A, H212RPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jip1 JBD
Plasmid#15751Purposebinds to JnkDepositorInsertJip1 (Jnk binding domain) (Mapk8ip1 Mouse)
UseTagsFlagExpressionMammalianMutationdeleted 1-126 and 282-660PromoterAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only