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Showing: 2621 - 2640 of 7291 results

  13. pJAK184.tetR.PT5-3.dCas9

    Plasmid
    #232482
    Purpose
    optimized ahTet inducible dCas9 for insertion into C. difficile genome
    Depositor
    James Collins
    Article
    Collins et al Microbiology Resource Announcements
    Insert
    Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TetR-PT5-3
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232482-plasmid-map-sequence-id-461406

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  14. pJC.15A.sgRNA.TA

    Plasmid
    #232483
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.
    Depositor
    James Collins
    Article
    Collins et al Microbiology Resource Announcements
    Inserts
    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3 constitutive promoter and native promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232483-plasmid-map-sequence-id-466415

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Showing: 2621 - 2640 of 7291 results