-
Plasmid#194221PurposeBackbone for constructing multi casette expression plasmids using the Mammalian TookitDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only
-
LCB1_v1.3 (MTK 3a) (pZYW044)
Plasmid#194227PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB1DepositorInsertLCB1_v1.3
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCB3_v1.2 (MTK 3a) (pZYW045)
Plasmid#194228PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB3DepositorInsertLCB3_v1.2
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
WPRE (MTK 4b) (pAN2410)
Plasmid#194235PurposeMammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbonesDepositorInsertWPRE terminator
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9
Plasmid#223163Purpose2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA ScaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG
Plasmid#217717PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR1 (CELSR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2453PromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-LBR(1-245)-GFP
Plasmid#222425PurposeTo express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cellsDepositorInsertLBR(1-245aa) (LBR Human)
UseTagsFKBP and GFPExpressionMammalianMutationTruncation (Includes aa 1-245)PromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-mCherry-RAB7-S72A
Plasmid#221556PurposeExpresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertRab7A (RAB7A Human)
UsePiggybacTagsmCherryExpressionMammalianMutationChange serine 72 to alaninePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)
Plasmid#221552PurposeExpresses Cas9 and gRNA for disruption of Rab7A gene in human cellsDepositorInsertgRNA targeting human Rab7A (RAB7A Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-KRAS-G121C/G13A-nick sgRNA
Plasmid#214099PurposeLentiviral vector expressing nicking sgRNA to induce KRAS G12C or G13A mutation using PE3DepositorInsertKRAS G12C or G13A nicking sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC1_oROS-HT
Plasmid#216413PurposeExpresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.DepositorInsertoROS-HT
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa VP64 Hbb
Plasmid#99693PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Synthetic, Mouse)
UseAAV and CRISPRTagsVP64ExpressionMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-EGFP
Plasmid#166137PurposeEGFP expression under the control of the GPD promoter yeastDepositorArticleInsertEGFP
UseTagsExpressionYeastMutationPromoterGDPAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-mRuby3
Plasmid#166138PurposemRuby3 expression under control of the GPD promoter in yeastDepositorArticleInsertmRuby3
UseTagsExpressionYeastMutationPromoterGDPAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pITESceIcmd
Plasmid#218288PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1DepositorInsertHO(-253, -1)-tSynth3UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40
Plasmid#216533PurposeAn axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switchDepositorInsertaxon-jGCaMP8m
UseAAV and Cre/LoxTagsGAP43ExpressionMammalianMutationPromoterhSynapsin1Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT365 AAV-Rapamycin Inducible-S3H
Plasmid#202066PurposeInducible AAV activatorDepositorArticleInsertAAV-Rapamycin Inducible-S3H
UseAAV; Aav with reporterTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT366 AAV-Rapamycin Inducible QNZF
Plasmid#202067PurposeInducible AAV activatorDepositorArticleInsertAAV-Rapamycin Inducible QNZF
UseAAV; Aav with reporterTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT310 AAV-BFPdonor
Plasmid#202057PurposeAAV HDR donorDepositorArticleInsertAAV-BFPdonor
UseAAV; Aav packaging plasmidTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGKCMV)-nucTomato-hygro
Plasmid#208573PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
d2nucEGFP
HygroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGK/CMV and minPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only