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Plasmid#86894Purposeexpress H74A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationW205APromoterT7Available sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-FLAGHISNDHFR
Plasmid#64001PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & FLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-FLAGNDHFR
Plasmid#63997PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-FLAGHISNDHFR
Plasmid#63965PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & FLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-hsp82
Plasmid#52909PurposeDrosophila pseudoobscura hsp82 promoter driving firefly luciferaseDepositorInsertDrosophila pseudoobscura hsp82 promoter
UseLuciferaseTagsExpressionMutationPromoterD pseudoobscura hsp82Available sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKDC.100
Plasmid#222224PurposeAcceptor plasmid for yeast retron-Eco1 ncRNA variant libraries.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM7073
Plasmid#214011PurposePrpsM driven expression of ssrA-tagged mCerulean (AAV)DepositorInsertmCerulean-AAV
UseTagsExpressionBacterialMutationPromoterPrpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS125
Plasmid#204593PurposeGST-lamin A C522A (389-646)DepositorInserthuman lamin A C522A (389-646)
UseTagsGST - lamin A C522A (389-646)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS38
Plasmid#204590PurposeGST-Progerin(506-664 delta 50)DepositorInserthuman progerin (506-664 delta 50)
UseTagsGST - progerin (506-664 delta 50)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL4624 (pEffector_TnpB1,ωRNA)
Plasmid#210870PurposeExpresses GstTnpB1 and native ωRNA from constitutive promotersDepositorInsertGstTnpB1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-GST-V5His6_E
Plasmid#146223PurposeInsect Expression of GSTDepositorInsertGST
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
KBB11
Plasmid#185422PurposeFull-length KAP60/SRP1DepositorInsertKAP60
UseTagsExpressionYeastMutationPromoterendogenousAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
TetO::EGFP-hMeikin
Plasmid#174705PurposeDoxycycline inducible expression of human MeikinDepositorInsertMeikin
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcrVIA4
Plasmid#164863PurposeInducible expression of AcrVIA4 in bacteria.DepositorInsertAcrVIA4
UseTagsExpressionBacterialMutationPromoterT7 PromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcrVIA7
Plasmid#164864PurposeInducible expression of AcrVIA7 in bacteria.DepositorInsertAcrVIA7
UseTagsExpressionBacterialMutationPromoterT7 PromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046691
Plasmid#149207PurposeYeast Expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046645
Plasmid#149174PurposeYeast Expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046657
Plasmid#149179PurposeYeast Expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046666
Plasmid#149167PurposeYeast Expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSADuet779
Plasmid#118395Purposeanalysis of cis-splicing by mini inteinsDepositorInsertMjaTFIIB mini intein
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only