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Plasmid#194250PurposeHuman RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterhuman rDNA promoter and beginning of 5' ETS …Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pChlamy/Cre
Plasmid#205440PurposeAn expression vector for Cre recombinase (codon usage for C. reinhardtii)DepositorInsertCre recombinase gene
UseChlamydomonas expressionTagsExpressionMutationPromoterHeat shock protein 70A (HSP70A)/ribulose bisphosp…Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL0527 (pDonor)
Plasmid#130634PurposeEncodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL0341 (pTarget)
Plasmid#200906PurposeEncodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.DepositorInsertpTarget
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL2783 (pTnsC-VP64_Vch)
Plasmid#200896PurposeExpresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.DepositorInsertVchTnsC-VP64
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL2669 (pTnsABf_Vch)
Plasmid#200897PurposeExpresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.DepositorInsertVchTnsA-TnsB
UseTagsInternal NLS between TnsA-TnsB fusionExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6
Plasmid#190162PurposeExpresses 24 Broccoli-tagged beta-actin mRNA in mammalian cellsDepositorInsertmcherry_(3'UTR, No Actin)_24Broccoli
UseTags24×BroccoliExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-Spy
Plasmid#90102PurposeIPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.DepositorInsertYcgR (ycgR )
UseBroad-host rangeTagsExpressionBacterialMutationPromoterPtacAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6T7G
Plasmid#71463PurposeExpresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and MammalianMutationPromoterhuman U6/phage T7Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pConRef-2H12
Plasmid#221182PurposecdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)DepositorInsertscdGreen
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIFV
Plasmid#111085PurposepNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectorsDepositorInsertempty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
UseDeleted foamy virusTagsExpressionMutationat nt 2334 A changed to T, at nt 2495 A changed t…PromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-F30-Squash-Broccoli
Plasmid#177918PurposeExpresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.DepositorInsertTornado-F30-Squash-Broccoli
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL0426 (CRISPR with spc3)
Plasmid#130642PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.DepositorInsertCRISPR(spacer-3)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK503
Plasmid#155380PurposedCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceMay 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMCS.71/2272
Plasmid#22732DepositorInsertLox71-MCS-Lox2272
UseCre/LoxTagsExpressionMutationThis is a basal exchange vector for RMCE containi…PromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMIG-Flag-RIPK1-wt
Plasmid#102677Purposeretroviral expression plasmid for RIPK1-wtDepositorInsertRIPK1 (RIPK1 Human)
UseRetroviralTagsFLAG and IRES-GFPExpressionMammalianMutationPromoterunknownAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hST3GAL6-RFP
Plasmid#22923DepositorInserthuman type 2 lactosamine alpha-2,3-sialyltransferase promoter driving DsRedExpress (ST3GAL6 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSL0373 (His-MBP-TniQ)
Plasmid#130638PurposeExpresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.DepositorInsertVchTniQ
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on TniQExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-Flag-TRAF5
Plasmid#102679Purposeretroviral expression plasmid for TRAF5DepositorInsertTRAF5 (Traf5 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBCC155-MXMT
Plasmid#79816PurposeCFP-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsCC155/CFP (155–239)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only