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Showing: 2601 - 2620 of 24604 results

  13. pSC-mCh-P2A-ST-EGFP-CAAX

    Plasmid
    #207636
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207636-plasmid-map-sequence-id-409876

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  16. pDA2ScIfm

    Plasmid
    #193786
    Purpose
    Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Frame-shifted CI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTATAA synthetic promoter carrying binding sites…
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    193786-plasmid-map-sequence-id-389837

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  17. pLA2ScIfmTcIfm

    Plasmid
    #193792
    Purpose
    Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLLacO-1 and PLTetO-1
    Available since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    193792-plasmid-map-sequence-id-390100

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  19. pLCHKO_HPRT1_exon3_1_As

    Plasmid
    #155057
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155057-plasmid-map-sequence-id-308520

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  20. pLCHKO_HPRT1_exon3_1_Lb

    Plasmid
    #155053
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155053-plasmid-map-sequence-id-308427

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Showing: 2601 - 2620 of 24604 results