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Plasmid#170769Purposetest plasmid for 5RM assembly, encodes fluorescent red proteinDepositorInserttagRFP
UseTagsExpressionMutationPromoternoneAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-79aa-ActA-pEGFP-C1
Plasmid#166763PurposeTo target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.DepositorInsertActA tail anchor (Listeria monocytogenes) (actA Listeria monocytogenes, Synthetic)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCell-Free_KA1_SARS-COV-2_S-frag3
Plasmid#169403PurposeCell-free/mammalian expression vector for SARS-COV-2 virus N-term fragment of S protein after the furin cleavag with N-term eGFPDepositorInsertN-term frag. S protein after the furin cleavag (N SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_S-frag5
Plasmid#169404PurposeCell-free/mammalian expression vector for SARS-COV-2 virus C-terminal ectodomain of S protein from the furin cleavag with N-term eGFPDepositorInsertC-term frag. S protein after the furin cleavag (S SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBHRSF63
Plasmid#118403Purposefor structure determinationDepositorInsertMvuTFIIB mini intein
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBHDuet33
Plasmid#118397Purposeanalysis of cis-splicingDepositorInsertMvuTFIIB intein
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-PvuRts1I
Plasmid#86887Purposeexpress N-term His tagged PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pBT317_pET-PhiC31o-pA
Plasmid#52491Purposefor preparation of PhiC31o mRNADepositorInsertPhiC31o integrase
UseTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2B-55
Plasmid#177924PurposeExpresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoterDepositorArticleInsertOptimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
UseTagsExpressionBacterial and YeastMutationV9I; F67S; G72P, F86Y; R171KPromoterTDH3Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2B-56
Plasmid#177925PurposeExpresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoterDepositorArticleInsertOptimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
UseTagsExpressionBacterial and YeastMutationV9I; F67S; G72P, F86Y; R171KPromoterMYO2Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEXY (pDN137)
Plasmid#72656Purposegolden gate entry vector for mCherry-LEXY tagging of proteins (no constitutive NLS)DepositorTypeEmpty backboneUseGolden gate entry vectorTagsmCherry-LEXYExpressionMammalianMutationPromoterCMV-FAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUGW-VGAT-pHluorin
Plasmid#207467PurposeLentiviral expression of VGAT-pHluorinDepositorArticleInsertVGAT (Slc6a12 Rat)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas9-CR4
Plasmid#62655PurposeCas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetRDepositorInsertCas9 nuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCWU6-xylR-E-luciferase
Plasmid#190721Purposefine tune expression of luciferase under the control of xylose- and theophylline- inducible systemDepositorInsertxylR-E-luciferase
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-Smad4
Plasmid#11743DepositorInsertSMAD4 (Smad4 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAD7-crRNA-2
Plasmid#170135PurposeFor crRNA expression in monocotyledons protoplastsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6_crRNA_mCherry
Plasmid#197609PurposeExpresses a Cas7-11 crRNA and mCherryDepositorInsertU6_crRNA_mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only