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Tol2-ISceI-ZSP:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194518

Purpose

ZSP promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam APC 1-1309

Plasmid

#16509

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Use

Tags

Expression

Mammalian

Mutation

Codon 1309 truncation

Promoter

Available since

April 30, 2008

Availability

Academic Institutions and Nonprofits only
pFUSEss-CHIg-mG2a_M18

Plasmid

#105930

Purpose

Expression plasmid coding for heavy chain of mouse IgG2a antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG2b heavy chain (Ighg2b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hEF1-HTLV prom

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBT372

Plasmid

#125613

Purpose

Expresses evoCDA1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoCDA1-BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

F23S A123V I195F

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Dsup pLVX-Puro

Plasmid

#180635

Purpose

Expresses human codon optimized tardigrade damage suppressing protein Dsup under a CMV promoter; to provide increased radio tolerance

Depositor

Christopher Mason

Article

Westover et al bioRxiv 2020.11.10.373571

Insert

Dsup

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-APOBEC1-YTH

Plasmid

#200158

Purpose

For bacterial expression of the APOBEC1-YTH fusion protein for protein purification

Depositor

Kate Meyer

Article

Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

Insert

APOBEC1-YTH

Use

Tags

6xHis, HA, and Maltose Binding Protein

Expression

Bacterial

Mutation

Codon optimized for E coli expression

Promoter

LacZ

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG mitoYFP

Plasmid

#168508

Purpose

Expression of mito-EYFP (mitochondrial matrix targeted EYFP) for visualization of mitochondrial dynamics

Depositor

Franck Polleux

Article

Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

Insert

mitoYFP

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMDISI

Plasmid

#51653

Purpose

E coli genetic modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

spcR

Use

Genetic modification

Tags

Expression

Mutation

Promoter

unknown

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pPSU50

Plasmid

#173832

Purpose

Expresses 50 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABMBP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam APC 1-1941

Plasmid

#16510

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Use

Tags

Expression

Mammalian

Mutation

Codon 1941 truncation

Promoter

Available since

April 30, 2008

Availability

Academic Institutions and Nonprofits only
pQUAST-attB

Plasmid

#104880

Purpose

For insertion of 5xQUAS-geneX transgenes using PhiC31 integrase system

Depositor

Christopher Potter

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ran-T24N-mRFP1-polyA

Plasmid

#104561

Purpose

Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran (Ran Mouse)

Use

Tags

mRFP1

Expression

Mammalian

Mutation

T24N

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPR-HOT-P2A-Clover-BlastR

Plasmid

#138568

Purpose

Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

Depositor

Hans Clevers

Article

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

P2A-clover-BlastR

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_BiVIPe4_ChR2_mCherry

Plasmid

#213856

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Nanog

Plasmid

#28221

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Jan 18. ():.

Insert

Nanog (NANOG Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2011

Availability

Academic Institutions and Nonprofits only

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ptrc99a-pVhb-tdTER-fdh

Plasmid

#215649

Purpose

pBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoter

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Inserts

trans-enoyl-CoA reductase
formate dehydrogenase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Vhb

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-4x

Plasmid

#124678

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAd-Track Flag HA PGC-1 alpha

Plasmid

#14426

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pBAD-Padron2

Plasmid

#172355

Purpose

Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

Depositor

Stefan Jakobs

Article

Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

Insert

Padron2

Use

Tags

6xHisTag (N terminal on backbone)

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-RNR2-1397N

Plasmid

#183897

Purpose

Expresses TALED_Right-RNR2-1397N in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

TALED for RNR2 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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