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Plasmid#183199PurposeEntry cloneDepositorInsertCas9-RFC4
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
KP501: pMVP (L5-L4) eYFP-P2A
Plasmid#121714PurposepMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.DepositorInserteYFP-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL106P-LUC-ENTR1A(w451-1)
Plasmid#17455PurposeGateway entry vector, Degron N-term LuciferaseDepositorInsertFKBP-Luciferase fusion (MTOR firefly)
UseLuciferase; Entry vectorTagsFKBP12DDExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
LF601: pMVP (L1-R5) TBG promoter
Plasmid#121695PurposepMVP L1-R5 entry plasmid, contains TBG promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertTBG promoter (Liver specific)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6-miR-30 R4-R3
Plasmid#62117PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0119
Plasmid#177051PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein+H34like (NmMLPL) from Nepeta mussinii driven by 35S promoterDepositorInsertmajor latex protein-like (NmMLPL) from Nepeta mussinii
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo:alpha4-mcherry
Plasmid#45073DepositorInsertalpha4-mcherry (Chrna4 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_K5S
Plasmid#98679PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5SDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationK1838S, K1859S, K1866S, K1873S, K1887SPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV L5-L2
Plasmid#62091PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
p15aC-3D1D-5
Plasmid#121050PurposeBacterial expression of PIS, PI4K and PI3P5K mutant E545KDepositorInsertsPIS
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 3-kinase catalytic subunit p110Ī± of class IA E545K
UseTagsmyc and noneExpressionBacterialMutationPromoterAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 P139A (#625)
Plasmid#99945Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationP139APromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 S137A (#627)
Plasmid#99943Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationS137APromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-OSBP
Plasmid#67677PurposeMultisite gateway entry clone for adding C-terminal fusions of OSBPDepositorInsertOSBP
UseGateway multisite 3' entry cloneTagsExpressionMutationaa 87-185 of NP_002547.1PromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pN1-mCherry-NKD2
Plasmid#176641PurposeExpresses NKD2-mCherry fusion protein in mammalian cellsDepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-3xFlag-S242R-MEFV
Plasmid#167020PurposeGateway entry plasmid with 3xFlag-pyrin (S242R/PAAND variant)DepositorInsertMEFV (MEFV Human)
UseGateway entry vectorTags3xFlagExpressionMutationserine 242 in arginine (S242R)PromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB02
Plasmid#111959PurposeExpresses GST-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
UseTagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monomeā¦PromoterT7Available sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
JN402: pMVP (R4-R3) Firefly Luciferase
Plasmid#121741PurposepMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertFirefly Luciferase
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3
Plasmid#162918PurposeGateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)DepositorInsertfirefly luciferase/green fluorescent protein fusion with upstream IRES sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
LC402: pMVP (L3-L2) eGFP + polyA
Plasmid#121763PurposepMVP L3-L2 entry plasmid, contains eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest.DepositorInserteGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KG802: pMVP (L3-L2) V5 epitope tag + polyA
Plasmid#121755PurposepMVP L3-L2 entry plasmid, contains V5 epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsertV5 epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only