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Plasmid#224477PurposeGateway compatible 5' entry clone containing a Lex Operon upstream of a c-fos minimal promoterDepositorInsertLexOp
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME V5-mTagBFP2 (JDW 830)
Plasmid#224484PurposeGateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)DepositorInsertV5-mTagBFP2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME nls EGFP-Cre (JDW 1329)
Plasmid#224515PurposeA Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusionDepositorInsertnls-EGFP-Cre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-mScarlet-I-P2A-iCre (JDW 917)
Plasmid#224516PurposeA Gateway compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized)DepositorInsertmScarlet-I-P2A-iCre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-3xNLS-mTagBFP2-Cre (JDW 918)
Plasmid#224517PurposeA Gateway compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusionDepositorInsert3xNLS-mTagBFP2-Cre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-mTurquoise-P2A-iCre (JDW 920)
Plasmid#224518PurposeA Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)DepositorInsertmTurquoise-P2A-iCre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME iCre-I (JDW 1203)
Plasmid#224520PurposeA Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteriaDepositorInsertiCre-I
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-H2A_mCherry_SV40pA (JDW 967)
Plasmid#224528PurposeA Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyADepositorInsertH2A-mCherry-SV40pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME H2B mCerulean (JDW 1150)
Plasmid#224543PurposeA Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleusDepositorInsertH2B-mCerulean
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Ark-L2
Plasmid#186362PurposeEntry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertCiona robusta Aurora kinase A
UseExpression of a fluorescent centrosome markerTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
Plasmid#186413PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.DepositorInsertlacZ (lacZ E. coli)
UseGateway destination vectorTagsNuclear localization signalExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB425
Plasmid#74819PurposeGateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-mVenus-CAAX
Plasmid#75153PurposeMembrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.DepositorInsertmVenus-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-mTagBFP-CAAX
Plasmid#75149PurposeMembrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.DepositorInsertmTagBFP-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVenusNfN
Plasmid#128564PurposeProtein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.DepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVenusCfC
Plasmid#128567PurposeProtein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.DepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVenusCfN
Plasmid#128566PurposeProtein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.DepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-mCherry-NS-MCS
Plasmid#75179PurposemCherry (red) fluorophore with multiple cloning site for use with inducible N-terminal fusions.DepositorInsertmCherry-NS-MCS
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-mKOFP2-CAAX
Plasmid#75155PurposeMembrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.DepositorInsertmKOFP2-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB321
Plasmid#68649PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only