-
Plasmid#183675PurposePtdIns(4,5)P2 biosensor for purification from bacteriaDepositorInsertPLCD1 (PLCD1 Human)
UseTagsHis6-EGFPExpressionBacterialMutationAmino acids 11-140PromoterT7Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
His6-SMT3-SopB
Plasmid#183676PurposeRecombinant Salmonella Typhimurium SopBDepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
UseTagsHis6-S. cerevisiae SMT3(2-101)ExpressionBacterialMutationPromoterT7Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
His6-SMT3-SopB(C460S)
Plasmid#183677PurposeRecombinant Salmonella Typhimurium SopB (catalytically inactive)DepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
UseTagsHis6-S. cerevisiae SMT3(2-101)ExpressionBacterialMutationC460SPromoterT7Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMJ841
Plasmid#39318DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationAspartate 10 to Alanine (D10A) and Histidine 840 …PromoterT7Available sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI Probe
Plasmid#211507PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteriaDepositorInsertBcPI-PLC-ANH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVSL10
Plasmid#201182PurposeBst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavableDepositorInsertBst polymerase large fragment (BF) F710Y/D598A
UseTagsHRV 3C protease cleavage site and His6ExpressionBacterialMutationF710Y D598APromoterT5-lacOAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHis-Laforin
Plasmid#157989PurposeExpresses Laforin in E.coli cellDepositorInsertLaforin (EPM2A Human)
UseTagsHis TagExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCJ136
Plasmid#162665PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ207
Plasmid#162680PurposepET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.DepositorInsertPutative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ199
Plasmid#162672PurposepET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.DepositorInsertPutative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ203
Plasmid#162678PurposepET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)DepositorInsertPutative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
UseTagsHisExpressionBacterialMutationdeltaF2-A75; codon optimized for expression in E.…PromoterT7/lacAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAP1-3
Plasmid#71258Purposeluciferase reporter with 3 copies of the stromelylisin gene AP-1 site in front of hGM-CSF -55 to +28 minimal promoterDepositorInsert3 copies of an AP-1 site from the human Stromelysin-1 gene (MMP3 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterGM-CSF minimalAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(4)P Probe
Plasmid#211509PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteriaDepositorInsertSidC (P4C)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(4,5)P2 Probe
Plasmid#211513PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteriaDepositorInsertPLCdelta1-PH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4,5)P3 Probe
Plasmid#211514PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteriaDepositorInsertBTK1-PH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,5)P2 Probe
Plasmid#211512PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteriaDepositorInsertSnxA-2xPX
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4)P2 Probe
Plasmid#211511PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteriaDepositorInsertTAPP1 cPHx3
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHL 802
Plasmid#52991PurposepLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfpDepositorInsertsOxyS sRNA
ompC
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutationcontains −81 to +36 bp relative to start codonPromoterpLlacO-1 and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 990
Plasmid#52992PurposepLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfpDepositorInsertsDsrA sRNA
RhyB sRNA (competitor)
rpoS
UseSynthetic BiologyTagsgfpExpressionBacterialMutationincludes −150 to +30 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1020
Plasmid#52995PurposepLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA (competitor)
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only