Addgene

We narrowed to 3,221 results for: ado

Showing: 241 - 260 of 3221 results

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pET11a-dH2B

Plasmid

#105501

Purpose

Expression of Drosophila histone H2B. An additional Ile codon was inserted after the initiating Met codon to enhance the protein expression level

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

dH2B

Use

Tags

Expression

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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IDP205 (T7->Rci; 5bc)

Plasmid

#68834

Purpose

Expresses Rci gene (driven by the inducible T7 promoter) and 5 fragment barcode cassette in bacterial cells

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2014;42(16):e127. doi

Inserts

Barcode cassette 5 fragments
Rci recombinase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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porcupine - Xenopus

Plasmid

#39772

Depositor

Norbert Perrimon

Article

Kadowaki et al Genes Dev. 1996 Dec 15;10(24):3116-28.

Insert

porcupine (porcn Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only
pEXP5-NT-AlleyCat

Plasmid

#41715

Depositor

Ivan Korendovych, William DeGrado

Article

Korendovych et al Proc Natl Acad Sci U S A. 2011 Apr 26;10

Insert

mutated C-terminal portion of calmodulin gene (M76-K148)

Use

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

F92E

Promoter

T7

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-GA

Plasmid

#191666

Purpose

ddGFP-A-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Use

Tags

HA and ddGFP-A

Expression

Mammalian

Mutation

Promoter

Chicken β-actin promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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SCP1-CMVenh-luc

Plasmid

#22216

Depositor

James Kadonaga

Article

Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

Insert

SCP1 (super core promoter 1)-CMVenh luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-B1

Plasmid

#191667

Purpose

ddFP-B1-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Use

Tags

HA and ddFP-B1

Expression

Mammalian

Mutation

Promoter

Chicken β-actin promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-V

Plasmid

#191665

Purpose

mVenus-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Use

Tags

HA and mVenus

Expression

Mammalian

Mutation

Promoter

Chicken β-actin promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-B3

Plasmid

#191668

Purpose

ddFP-B3-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Use

Tags

HA and ddFP-B3

Expression

Mammalian

Mutation

Promoter

Chicken β-actin promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRMCD30

Plasmid

#173033

Purpose

pQE30 with S. flexneri 2a wzzBSF gene

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

S. flexneri 2a wzzBSF gene

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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porcupine

Plasmid

#37377

Depositor

Norbert Perrimon

Article

Kadowaki et al Genes Dev. 1996 Dec 15;10(24):3116-28.

Insert

porcupine (por Fly)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
IDP456

Plasmid

#87002

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

barcode landing site-mCherry-4xboxB

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-RBM3

Plasmid

#87860

Purpose

Expresses human RBM3 coupled to GFP

Depositor

Maria Alvarado-Kristensson

Article

Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle

Insert

RNA binding motif protein 3 (RBM3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

NA

Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP9 MLH1

Plasmid

#16458

Depositor

Bert Vogelstein

Article

Papadopoulos et al Science. 1994 Mar 18. 263(5153):1625-9.

Insert

MLH1 (MLH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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SCP2-CMVenh-luc

Plasmid

#22230

Depositor

James Kadonaga

Article

Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

Insert

SCP2 (super core promoter 2)-CMVenh luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pAGD236

Plasmid

#219497

Purpose

pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

sfGFP, Eco1 msr, Eco1 RT

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAGD248

Plasmid

#219498

Purpose

Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pSP6-EBNA(2A+DBD)

Plasmid

#98749

Purpose

In vitro transcription of modified EBNA1 mRNA

Depositor

Sara Howden, Jim Vadolas

Article

Howden et al Hum Gene Ther. 2006 Aug;17(8):833-44.

Insert

Modified EBNA1

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

SP6

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo-hCG

Plasmid

#16574

Depositor

Bert Vogelstein

Article

Shih et al Nat Med. 2000 Jun . 6(6):711-4.

Insert

Beta-hCG (CGB3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pET12a-AL-103 FL

Plasmid

#47090

Depositor

Marina Ramirez-Alvarado

Article

Martin et al Amyloid. 2010 Sep;17(3-4):129-36. doi: 1

Insert

AL-103 (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only