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Plasmid#208053PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.DepositorTypeEmpty backboneUseTagsTwin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only
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pET24b-BG1.3
Plasmid#199437Purposeexpresses BAmGreen1.3 in bacterial cellsDepositorInsertBAMGreen1.3
UseTagshis tagExpressionBacterialMutationPromoterT7Available sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-5'TwStrep
Plasmid#208620PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.DepositorTypeEmpty backboneUseTagsTwin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a-C2-SNAP
Plasmid#208769PurposeTo express C2-SNAP in bacteriaDepositorInsertC2-SNAP (Mfge8 Mouse)
UseTagsSNAP-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a-C2-mKate
Plasmid#208763PurposeTo express C2-mKate in bacteriaDepositorInsertC2-mKate (Mfge8 Mouse)
UseTagsmKateExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PACT
Plasmid#50352Purposebacterial expression of PACTDepositorInsertPACT (PRKRA Human)
UseTagsHis and MycExpressionBacterialMutationPromoterT7Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET23b-NTF2
Plasmid#108918PurposeExpresses NTF2DepositorInsertNTF2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a-LIC
Plasmid#62303PurposeE. coli expression vector with N-terminal Hexa-HIS and TEV cleavageDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
eSrtA(2A-9) pET29b
Plasmid#75145Purposereprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificityDepositorInsertreprogrammed sortase A variant
UseTags6x HisExpressionBacterialMutationS102C A104H E105D K138P K152I N160K K162H T164N K…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-His6_pET21a
Plasmid#107178Purposeexpresses His6-tagged miniTurbo in bacteriaDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionBacterialMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI
Plasmid#50723Purposeoverexpression of human TPI1 in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-At-TRL
Plasmid#32242DepositorInsertA. thaliana tRNA ligase (RNL Mustard Weed)
UseTags6xHistidineExpressionBacterialMutationPromoterT7 RNA PolmyeraseAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-mtLeuRS
Plasmid#31079DepositorInsertMature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
UseTagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Nt …PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
BglB in pET29b(+)
Plasmid#55773PurposeExpresses beta-glucosidaseDepositorInsertBeta-glucosidase B (bglB Synthetic)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a MISTIC
Plasmid#85999Purposebacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tagDepositorInsertN/A
UseTagsHIS6 and MISTICExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-gp32-H6
Plasmid#163912PurposeExpresses T4 gp32 for bacterial expression and affinity purificationDepositorInsertgp32
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP
Plasmid#166439PurposeBacterial expression of 6x His tagged mEGFPDepositorInsertmEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-uvsX-H6
Plasmid#163913PurposeExpresses uvsX for bacterial expression and affinity purificationDepositorInsertuvsX
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-KIRIN1
Plasmid#107414PurposeBacterial Expression plasmid for potassium ion raitometric indicator KIRIN1DepositorInsertKIRIN1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6HuR
Plasmid#135943PurposeExpresses HuR in E. coliDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only