Addgene

We narrowed to 7,978 results for: 027

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His-ATG3-R265A

Plasmid

#202783

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Tags

6xHis, TEV

Expression

Bacterial

Mutation

R265A

Promoter

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-H266A

Plasmid

#202784

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Tags

6xHis, TEV

Expression

Bacterial

Mutation

H266A

Promoter

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-K62D/K64D

Plasmid

#202786

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K62D/K64D-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Tags

6xHis, TEV

Expression

Bacterial

Mutation

K62D, K64D

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCFD3-Act5c

Plasmid

#130278

Purpose

Drosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

pCFD3-Act5c (Act5C Fly)

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00199

Plasmid

#34350

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10274 (CG10274 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KSHV Tiling Library

Pooled Library

#180272

Purpose

sgRNA library tiling entire ~154 kb of the BAC16 Kaposi’s Sarcoma-associated Herpes viral genome. A guide every ~8 bp allows pooled screening of viral genome.

Depositor

Britt Glaunsinger

Article

Morgens et al PLoS Pathog. 2022 Jan 18;18(1):e1010236.

Expression

Mammalian

Species

Kaposi’s sarcoma-associated herpes virus

Use

Lentiviral

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only
pcDNA-Flag-Kdm6b(1025-End)-pA

Plasmid

#100278

Purpose

In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

Depositor

Yi Zhang

Article

Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

Insert

Kdm6b (Kdm6b Mouse)

Use

Tags

Flag epitope tag

Expression

Mammalian

Mutation

catalytic domain (1025aa-End)

Promoter

T7

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

Plasmid

#100279

Purpose

In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

Depositor

Yi Zhang

Article

Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

Insert

Kdm6b (Kdm6b Mouse)

Use

Tags

Flag epitope tag

Expression

Mammalian

Mutation

Mutated catalytic domain (1025aa-End) (please see…

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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sgp27#1

Plasmid

#102759

Purpose

Expresses sgRNA targeting mouse p27

Depositor

Jin Chen

Article

Song et al Oncogene. 2017 Jun 5. doi: 10.1038/onc.2

Insert

p27 (Cdkn1b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCFD3-Act5c

Plasmid

#130278

Purpose

Drosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

pCFD3-Act5c (Act5C Fly)

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-JAG2-Fc(DAPA)-AviTag-6xHis

Plasmid

#157027

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

JAG2 (JAG2 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm6b(1025-End)-pA

Plasmid

#100278

Purpose

In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

Depositor

Yi Zhang

Article

Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

Insert

Kdm6b (Kdm6b Mouse)

Use

Tags

Flag epitope tag

Expression

Mammalian

Mutation

catalytic domain (1025aa-End)

Promoter

T7

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

Plasmid

#100279

Purpose

In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

Depositor

Yi Zhang

Article

Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

Insert

Kdm6b (Kdm6b Mouse)

Use

Tags

Flag epitope tag

Expression

Mammalian

Mutation

Mutated catalytic domain (1025aa-End) (please see…

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
sgp27#1

Plasmid

#102759

Purpose

Expresses sgRNA targeting mouse p27

Depositor

Jin Chen

Article

Song et al Oncogene. 2017 Jun 5. doi: 10.1038/onc.2

Insert

p27 (Cdkn1b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCGN-MEF2B

Plasmid

#32959

Purpose

For mammalian expression of human MEF2B with an HA tag

Depositor

Ron Prywes

Article

Han et al Mol Cell Biol. 1995 Jun;15(6):2907-15.

Insert

MEF2B (MEF2B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

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FH95pUP95E17RGW (D4)

Plasmid

#74027

Purpose

lentiviral expression of Psd95 shRNA and Psd95-Q15A-EGFP fusion

Depositor

Weifeng Xu, Robert Malenka, Oliver Schluter

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

E17R

Promoter

H1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pD649-HAsp-JAG2-Fc(DAPA)-AviTag-6xHis

Plasmid

#157027

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

JAG2 (JAG2 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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Act5C-pDONR221

Plasmid

#124242

Purpose

Gateway entry clone of Drosophila Actin5C cDNA.

Depositor

Peter Vilmos

Insert

Drosophila Actin 5C (Act5C Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK RBJ 3'UTR

Plasmid

#19866

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

RBJ 3'UTR (DNAJC27 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00317

Plasmid

#34442

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10270 (D19B Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only