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Addgene
Showing: 601 - 650 of 867 results

  1. CyTERM-mGFP

    Plasmid
    #62237
    Purpose
    ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.
    Depositor
    Erik Snapp
    Article
    Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1
    Insert
    CyTERM-mGFP
    Use
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    signal anchor of rabbit cytochrome p450 and monom…
    Promoter
    CMV
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    62237-plasmid-map-sequence-id-320621

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  8. pBALU9

    Plasmid
    #69324
    Purpose
    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOP
    Depositor
    Oliver Hobert
    Article
    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/
    Insert
    gSL2-NLS-GFPint-FgF
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pBALU12

    Plasmid
    #69328
    Purpose
    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd mChO intron;use C-term after STOP
    Depositor
    Oliver Hobert
    Article
    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/
    Insert
    gSL2-NLS-mChOint-FgF
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pBALU18

    Plasmid
    #69334
    Purpose
    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2ndmChO intron; use C-term after STOP
    Depositor
    Oliver Hobert
    Article
    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/
    Insert
    rSL2-NLS-mChOint-FgF
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. dSpCas9-VPR

    Plasmid
    #205247
    Purpose
    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.
    Depositor
    Rasmus Bak
    Article
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSpCas9-VPR
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    205247-plasmid-map-sequence-id-408440

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  23. pcDNA3.1-Flag-gp130dimer

    Plasmid
    #174394
    Purpose
    Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).
    Depositor
    Dennis Ko
    Article
    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-
    Insert
    IL6ST cytosolic domain with leucine zipper & C-C linker
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174394-plasmid-map-sequence-id-341883

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  29. dSaCas9-VPR

    Plasmid
    #205251
    Purpose
    In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495
    Depositor
    Rasmus Bak
    Article
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSaCas9-VPR
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    205251-plasmid-map-sequence-id-408439

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  30. pLXIN-Ap1g1-FKBP

    Plasmid
    #46946
    Purpose
    Retroviral plasmid for co-expressing siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cells
    Depositor
    Margaret Robinson
    Article
    Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:
    Insert
    Ap1g1-FKBP
    Use
    Retroviral
    Tags
    Mainly mouse Ap1g1 cDNA, with a fragment of brain…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46946-plasmid-map-sequence-id-66695

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Showing: 601 - 650 of 867 results