Addgene

We narrowed to 4,156 results for: Lta;

Showing: 241 - 260 of 4156 results

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MTF1 deltaC18

Plasmid

#205070

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

MTF1 (MTF1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks C-terminal 18 amino acids

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MTF1 deltaC20

Plasmid

#205071

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

MTF1 (MTF1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks C-terminal 20 amino acids

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-TdTdelta5cys

Plasmid

#207465

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variant

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTdelta5cys (Dntt Mouse)

Use

Tags

10xHis

Expression

Bacterial

Mutation

Cys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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MTF1 deltaC9

Plasmid

#205067

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

MTF1 (MTF1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks C-terminal 9 amino acids

Promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)

Plasmid

#197969

Purpose

Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

L1332V, delta(1616-1666)

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-CLCdeltaN

Plasmid

#193562

Purpose

Expresses human Clathrin Light Chain B truncation mutant (deleted 1-89 aa) tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Deleted amino acids 1-89

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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His8OphMADeltaC6-18mer.

Plasmid

#189671

Purpose

His8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptides

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMA∆C6-TEV18mer

Use

Tags

His8 tagged

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-Mcl1_deltaTM

Plasmid

#187118

Purpose

insect cell expression of Mcl1-deltaTM

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

MCL-1 (BCL2 Human)

Use

Tags

Strep

Expression

Insect

Mutation

delta328-350

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-DDIT4_delta8

Plasmid

#187117

Purpose

insect cell expression of DDIT4-delta1-8

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

DDIT4 (DDIT4 Human)

Use

Tags

Strep

Expression

Insect

Mutation

delta1-8

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6.3delta4 GPR3-V5

Plasmid

#172601

Purpose

Lentiviral plasmid expressing c-terminal V5-tagged GPR3

Depositor

Robert Screaton

Article

Iorio et al Commun Biol. 2021 Jul 23;4(1):907. doi:

Insert

G protein-Coupled Receptor 3 (GPR3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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deltaLoop-MIDAS

Plasmid

#171605

Purpose

Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)

Use

Tags

His6 tag and SNAP tag

Expression

Bacterial

Mutation

Replaced amino acids 4458-4496 with ASGSGS linker

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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Mdn1-deltaC

Plasmid

#171603

Purpose

Expresses S. pombe Mdn1 a.a. 1-3911 in insect cells.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

Mdn1

Use

Tags

His6 tag

Expression

Insect

Mutation

C-terminal deletion; a.a. 1-3911 only

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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deltaVP1 only

Plasmid

#166675

Purpose

Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Insert

Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Oct4deltaPE-53BP1mCherry

Plasmid

#141122

Purpose

Construct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cells

Depositor

John Schimenti

Article

Bloom et al Genesis. 2020 Aug;58(8):e23368. doi: 10.

Insert

Fragment of human 53BP1 (TP53BP1 Human)

Use

Mouse Targeting

Tags

mCherry

Expression

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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MtMscL delta49

Plasmid

#118342

Purpose

M. tuberculosis MscL (delta49) in pET19b

Depositor

Douglas Rees

Article

Herrera et al Sci Rep. 2018 Oct 1;8(1):14566. doi: 10.

Insert

MscL (mscL Mycobacterium tuberculosis)

Use

Tags

6x His Tag and Thrombin Cleavage site

Expression

Bacterial

Mutation

Changed Glutamic Acid (Glu) 102 to Stop Codon

Promoter

T7 Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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SLAC-deltaC

Plasmid

#117102

Purpose

protein expression in yeast

Depositor

Geoffrey Chang

Insert

SLAC-deltaC

Use

Tags

His

Expression

Yeast

Mutation

Promoter

AOX

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only
SLAC1-deltaN

Plasmid

#117101

Purpose

protein expression in yeast

Depositor

Geoffrey Chang

Insert

SLAC1-deltaN

Use

Tags

His

Expression

Yeast

Mutation

Promoter

AOX

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37deltaSEP

Plasmid

#113501

Purpose

Bacterial expression of GST-tagged p37 lacking the SEP domain

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Use

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 140-206

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37delta2-81

Plasmid

#113502

Purpose

Bacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Use

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 2-81

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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DeltaNIRD KVS-1

Plasmid

#113069

Purpose

Expresses worm truncated KVS1.2 in mammalian cells

Depositor

Federico Sesti

Article

Cai et al J Biol Chem. 2007 Jun 22;282(25):18597-6

Insert

K+ voltage sensitive channel subunit 1.2 (kvs-1 Nematode)

Use

Tags

Expression

Mammalian

Mutation

first 40 AA deletion

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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