3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

Bacterial expression of Pfu DNA polymerase

Red fluorescent protein-based glucose sensor for mammalian cells with higer affinity

Mammalian expression of large NanoBit fragment (LgBit)

CRISPR array plasmid for NOTCH2 (Spacers 25-48)

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons

Gluc exon 2 with 50bp AAVS1 homology arms

The most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.

Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.

Red fluorescent protein-based glucose sensor for mammalian cells with lower affinity

Targeted CpG methylation

3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome

5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene