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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 36399 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX_3
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 15045
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVSV Genome
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Insert Size (bp)12000
- Promoter VSV promoters
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer ATGTCT GTTACAGTCA AGAGAATC (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid could potentially be used to generate BL2 virus. It is the responsibility of the recipient scientist to ensure that they are in compliance with their institutions’ biosafety requirements.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVSV Venus VSV-G was a gift from Connie Cepko (Addgene plasmid # 36399 ; http://n2t.net/addgene:36399 ; RRID:Addgene_36399) -
For your References section:
Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors. Beier KT, Saunders A, Oldenburg IA, Miyamichi K, Akhtar N, Luo L, Whelan SP, Sabatini B, Cepko CL. Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15414-9. Epub 2011 Aug 8. 10.1073/pnas.1110854108 PubMed 21825165