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Plasmid#140729PurposeOsTIR1(WT)-P2A-mAID-EGFP-NESDepositorInsertOsTIR1(WT)-P2A-mAID-EGFP-NES
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1/Pur
Plasmid#171682Purposeconstitutive expression of TIR1-myc in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertOsTIR1-MYC
UseTagsMYCExpressionMammalianMutationPromoterEF-1a promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-osTIR1-9myc
Plasmid#182043Purposein vitro transcription of osTIR1DepositorInsertosTIR1
UseIn vitro transcriptionTagsmycExpressionMutationPromoterAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KJ101: pMVP (R4-R3) osTir1
Plasmid#121742PurposepMVP R4-R3 entry plasmid, contains osTir1 for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertosTir1
UseSynthetic Biology; Pmvp gateway entry plasmidTags9x myc epitope tagExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PLC-TIR-GFP
Plasmid#52736PurposeRetroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and PLCd1 Lipid bindingExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-Tir
Plasmid#221326PurposeDox-inducible expression of Tir in mammalian cells by retroviral transductionDepositorInsertTir
UseRetroviralTagsHN-FLAGExpressionMutationPromoterTRE3GVAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SLP2a-TIR-GFP
Plasmid#52738PurposeRetroviral vector expresses the lipid binding domain of SLP2a fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsC2A domain of Murine SLP2a and GFPExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMK444 (EF1-OsTIR1(F74G) Puro)
Plasmid#214374PurposeEF1-OsTIR1(F74G) (PiggyBac)DepositorInsertEF1-OsTIR1(F74G)
UseTagsExpressionMammalianMutationPromoterEF1 promoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK395 (AAVS1 CMV-OsTIR1(F74A))
Plasmid#159992PurposeExpression of OsTIR1(F74A) in human cellsDepositorInsertCMV-OsTIR1(F74A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only