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Plasmid#72824PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Neo
UseCRISPRTagsmAIDExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1_Ec_rpoZ
Plasmid#128837PurposeE. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.DepositorInsertrpoZ (rpoZ E. coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-EEA1 wt
Plasmid#42307DepositorInsertEEA1 (EEA1 Human)
UseTagsGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-Cre-blast
Plasmid#179390PurposeLentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.DepositorInsertmCherry (P2A) Cre recombinase
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7f-WPRE (AAV9)
Viral Prep#104488-AAV9PurposeReady-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP7f-WPRE (#104488). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7f-WPRE plasmid DNA. Synapsin-driven GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry2-C1
Plasmid#54563PurposeLocalization: C1 Cloning Vector, Excitation: 587, Emission: 610DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsmCherry2ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
NFAT luciferase reporter
Plasmid#10959DepositorInsertIL-2 promoter (IL2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T2
Plasmid#41818PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (AAV5)
Viral Prep#84446-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (#84446). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] plasmid DNA. CAG-driven, Cre-dependent expression of Jaws-KGC-tdTomato-ER2 for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cre:GFP
Plasmid#13776PurposeMammalian expression of Cre recombinase fused to GFPDepositorInsertCre-GFP fusion protein
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pG3H-PE3max-attR1R2
Plasmid#213049PurposeDestination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sitesDepositorInsertsCas9 nickase
M-MLV Reverse Transcriptase
UseNote: this plasmid needs helper pvs1-vir2 for rep…TagsExpressionPlantMutationH840A, R240K, N413K,PromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-TOP-dGFP-reporter
Plasmid#14715PurposeBeta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).DepositorInsertLEF-1/TCF-responsive promoter (Ctnnb1 )
UseLentiviralTagsd2 eGFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
FUdeltaGW-rtTA
Plasmid#19780Purpose3rd generation lentiviral vectorDepositorInsertreverse tetracycline transactivator
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (AAV1)
Viral Prep#198511-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (#198511). In addition to the viral particles, you will also receive purified pAAV_hSyn-DIO-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven Cre-dependent expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmScarlet (Cre-dependent)Available sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH N90-beta-catenin
Plasmid#86499PurposeMammalian expression of Myc tagged N90 beta-cateninDepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsMycExpressionMammalianMutationDeletion of amino acids 1-90; constitutively acti…PromoterEF1aAvailable sinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA
Plasmid#183776PurposeAAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.DepositorInsertCre
UseAAVTagsExpressionMutationNAPromoterCAGAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-s ONE-GO
Plasmid#189731PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 GFP shRNA
Plasmid#30323DepositorInsertGFP shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) (AAV5)
Viral Prep#121539-AAV5PurposeReady-to-use AAV5 particles produced from pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) (#121539). In addition to the viral particles, you will also receive purified pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) plasmid DNA. Synapsin-driven, HA-tagged hM3D(Gq) for neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsHAAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CA
Plasmid#116771PurposeLentiviral expression of PIK3CADepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only