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Plasmid#53066Purposedestination vector with cytosol marker (ECFP) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only
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vPyACR_2164382
Plasmid#153029PurposeAnion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).DepositorInsertvPyACR_2164382
UseTagseYFPExpressionMammalianMutationvPyACR_2164382 was C-terminally truncated to incl…PromoterCMV (+ enhancer)Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
vPyACR_21821
Plasmid#153030PurposeAnion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).DepositorInsertvPyACR_21821
UseTagseYFPExpressionMammalianMutationvPyACR_21821 was C-terminally truncated to includ…PromoterCMV (+ enhancer)Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
YF-AIP
Plasmid#61525PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with EYFP and AIPDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP and EYFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cyt-n
Plasmid#53065Purposedestination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-er-n
Plasmid#53067Purposedestination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I affinity mutant (F40-based tension sensor)
Plasmid#119190PurposeThe affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.DepositorInserthuman Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
AURKA-Aquamarine
Plasmid#228564PurposeEncodes a donor-only, Aquamarine-tagged version of the AURKA biosensor under the control of a CMV promoterDepositorUseTagsAquamarineExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-q-CFP
Plasmid#66081PurposeThis G protein alpha-q construct contains internal insertions of CFP and the EE epitopeDepositorInsertG protein alpha-q-EE-CFP (Gnaq Mouse)
UseTagsCFP was inserted internally into the alpha-q sequ…ExpressionMammalianMutationBamHI and SacI sites in alpha-q were removed with…PromoterCMVAvailable sinceJuly 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-PEmax-IRES-ZeoR
Plasmid#207353PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.DepositorInsertPrime editor max (PEmax) enzyme
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYEE
Plasmid#159750PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238)-beta-5 in pcDNAI/Amp
Plasmid#55763PurposeA carboxyl-terminal CFP fragment was fused to Gbeta-5. When co-expressed with an amino terminal CFP or YFP fragment fused to a Ggamma subunit or RGS7, a fluorescent signal is produced.DepositorInsertCFP(159-238)-beta-5 (GNB5 Aequorea victoria, Human)
UseTagsCFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationCFP(159-238) includes a substitution of His for A…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
S10A mutant of H3S10ph biosensor
Plasmid#120810PurposeFRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capabilityDepositorInsertMouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
UseTagsExpressionMammalianMutationSerine 10 is mutated to Alanine, and Threonine 3,…PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-PE2-IRES-ZeoR
Plasmid#207352PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.DepositorInsertPrime editor 2 (PE2) enzyme
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238)-gamma-2 in pcDNAI/Amp
Plasmid#55777PurposeA C-terminal CFP fragment was fused to Ggamma-2. When co-expressed with an N-terminal mCerulean, CFP, or YFP fragment fused to a Gbeta subunit with which it interacts a fluorescent signal is produced.DepositorInsertCFP(159-238)-gamma-2 (GNG2 Aequorea victoria, Human)
UseTagsCFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationCFP(159-238) includes a substitution of His for A…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-MLH1dn-IRES-puroR
Plasmid#207354PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).DepositorInsertMutL homolog 1 (MLH1 Human)
UseLentiviralTagsExpressionMutationΔ754-756 dominant negative mutantPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238)-beta-1 in pcDNAI/Amp
Plasmid#55592PurposeA carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertCFP (159-238)-Beta 1 (GNB1 Aequorea victoria, Human)
UseTagsCFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationCFP(159-238) includes a substitution of His for A…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL 1951
Bacterial Strain#69773PurposeMG1655 + pLacO-1::T710:::YFP at GalKDepositorBacterial ResistanceNoneAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
HL 6686
Bacterial Strain#69775PurposeMG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + ΔdamDepositorBacterial ResistanceNoneAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
HL 3500
Bacterial Strain#69774PurposeMG1655 + pLacO-1::T710:::YFP at GalK + ΔLacIDepositorBacterial ResistanceNoneAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only