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Plasmid#184872PurposeChromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
SLF060: Lenti-CaMKIIa-jRGECO1a
Plasmid#112282PurposeFor lentiviral or direct expression of cytosolic jRGECO1a in excitatory neuronsDepositorInsertjRGECO1a
UseLentiviralTagsNES-6xHISExpressionMammalianMutationPromoterCaMKIaAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2-LSSmOrange
Plasmid#118856Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-LSSmOrange
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…PromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pvimentin-miRFP713
Plasmid#136569PurposeVimentin labeling with near-infrared fluorescent protein miRFP713DepositorInsertvimentin-miRFP713
UseTagsmiRFP713ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
JH03
Plasmid#73512PurposemKate2_PH pCFJ150 - contains C. elegans codon optimized mKate2DepositorInsertpmex-5::mKate2::PH::tbb-2
UseTagsmKate2ExpressionMutationPromotermex-5Available sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
AL12R-HSP70
Plasmid#161010PurposePP7-tagged reporter driven by the Arabidopsis HSP70 promoterDepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
mPlum-CENPB-N-22
Plasmid#55957PurposeLocalization: Nucleus/Centromeres, Excitation: 590, Emission: 649DepositorInsertCENPB (CENPB Human)
UseTagsmPlumExpressionMammalianMutationaa 1-169 of CENPB (DNA Binding Domain)PromoterCMVAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
TIGRE-TRE-[poor kozak]Bxb1-ecDHFR-BGHpA
Plasmid#158390PurposeInducible Bxb1 integrase for mammalian cellsDepositorInsertBxb1-ecDHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV LDTS-LIRmut
Plasmid#189001Purposelipophagy-inducing adaptor protein, negative controlDepositorInsertLDTS-LIRmut
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-JEDI-1P
Plasmid#202607PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)DepositorInsertJEDI-1P
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4L S382A S468A
Plasmid#27003DepositorInsertNEDD4L (NEDD4L Human)
UseTagsHAExpressionMammalianMutationS382A S468APromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
Tom20-CIB-stop
Plasmid#117243PurposeLight activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switchDepositorInsertCIBN
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
smHA multi-frame tag
Plasmid#128611Purposea spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.DepositorInsertsmHA MF tag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-Smad3 WT
Plasmid#27010DepositorInsertSmad3 (SMAD3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-Tubulin-C-18
Plasmid#57160PurposeLocalization: Microtubules, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertTubulin (TUBA1B Human)
UseTagsmPA-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pF3BGX-T2A-GAL4-DBD
Plasmid#138394PurposeRMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)DepositorInsertT2A-GAL4-DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
JAB2098
Plasmid#190340PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pGL3-p27Luc
Plasmid#23047DepositorInsertp27 (CDKN1B Human)
UseTagsLuciferaseExpressionMammalianMutationwt cDNAPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD
Plasmid#162804PurposeSTELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integrationDepositorInsertUb-BsmBI_IRES_Blasticidin
UsePiggybacTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only