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Showing: 2561 - 2580 of 57057 results

  2. pGG442

    Plasmid
    #165616
    Purpose
    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    Use
    CRISPR
    Tags
    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165616-plasmid-map-sequence-id-330958

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  6. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

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  10. pGM202T7

    Plasmid
    #69993
    Purpose
    E. coli/Streptomyces expression vector containing the T7-promoter
    Depositor
    Günther Muth
    Article
    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(
    Inserts
    aphII
    tsr
    rep
    sso
    T7 promoter
    dso
    His-Orf
    Use
    Tags
    N-terminal and C-terminal His tag encoding sequen…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    69993-plasmid-map-sequence-id-129665

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  19. pEGFPN1‐mTim1

    Plasmid
    #49215
    Purpose
    expresses EGFP tagged mouse Tim1
    Depositor
    Lawrence Kane
    Insert
    Tim1 (Havcr1 Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 2561 - 2580 of 57057 results