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Plasmid#207764PurposeDonor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorArticleInsertTUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2
Plasmid#203229PurposeCre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.DepositorInsertCre-dependent, membrane-localized Optopatch
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-OPTN (2-119)
Plasmid#208867PurposeStably express GFP-tagged OPTN (2-119aa) for CID induced mitophagyDepositorInsertOPTN (OPTN Human)
UseTagsFKBP-GFPExpressionMammalianMutationaa 2-119 onlyPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPolI_SARS-CoV-2_NLuc
Plasmid#183953PurposeEncodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoterDepositorUseTagsExpressionMammalianMutationPromoterMammalian Pol IAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_g5-HT2h
Plasmid#208709PurposeExpresses the green 5-HT sensor GRAB_g5-HT2h in mammalian cellsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2h
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE TurboID-HA donor (all-in-one) ORF0
Plasmid#200383PurposeTagging endogenously expressed proteins with TurboID-HA, C-terminalDepositorInsertTurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE TurboID-HA donor (all-in-one) ORF2
Plasmid#200385PurposeTagging endogenously expressed proteins with TurboID-HA, C-terminalDepositorInsertTurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_g5-HT3.0
Plasmid#208711PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in mammalian cellsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CMV_oROS-G
Plasmid#216111PurposeExpresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.DepositorInsertoROS-G
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_g5-HT3.0
Plasmid#208727PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytesDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVTagsExpressionMutationPromoterGfaABC1DAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV2_CAG_oROS-G
Plasmid#216115PurposeEncodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.DepositorInsertoROS-G
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK)
Plasmid#172328PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGKAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_r5-HTmut
Plasmid#208720PurposeExpresses the red 5-HT sensor GRAB_r5-HTmut in neuronsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HTmut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-ACTB
Plasmid#207748PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of ACTB (ACTB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TetON-Bmpr1A-ca
Plasmid#217536PurposeAAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptorDepositorArticleInsertconstitutively active BMP receptor 1a (BMPR1A Human)
UseAAVTagsHAExpressionMammalianMutationPromoterTet operon (TetO)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAC_DARIC(2-3)_CD22-BBz_AAV6_Donor
Plasmid#211906PurposeVector for AAV6 donor production. Targeted CD22-BBz DARIC transgene integration to the TRAC locus via homology-directed repair.DepositorInsertDARIC-CD22-BBz
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP - FLAG-APEX2- hs Lamin B1
Plasmid#139442PurposeAPEX2-Lamin B1 expression plasmidDepositorInsertLMNB1 (LMNB1 Human)
UseTags3xGGGGS linker (between APEX2 and Lamin B1), APEX…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-GRAB_g5-HT3.0
Plasmid#208723PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in excitatory neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVTagsExpressionMutationPromoterCaMKIIAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
ITPKA-mTurquoise2
Plasmid#137811PurposeIn vivo visualization of actin cytoskeleton (can be used for colocalization studies)DepositorArticleInsertITPKA (ITPKA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)-GtwyA
Plasmid#208574PurposeLV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
HSVtk
Neomycin
d2nucEGFP
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGK/CMV and minPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only