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Plasmid#181830PurposeGenetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.DepositorInsertLyn-PlcR
UseTagsECFP, EYFP, and N-terminal targeting sequence fro…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET FlipSuc-90u delta1
Plasmid#14475DepositorInsertFlipThuE W283A
UseTagsECFP and EYFPExpressionBacterialMutationN192D, W283A, 13 amino acids deleted from linkersPromoterT7Available sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAL101
Plasmid#22474PurposeExpresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to PhyDepositorInsertPIF6APB
UseSynthetic BiologyTagsmYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFLIPglu-2uDelta13
Plasmid#12990DepositorInsertFLIPglu F16F D152A Delta13
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationD152A mutation in binding proteinPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFLIPglu-600uDelta13
Plasmid#12991DepositorInsertFLIPglu F16A Delta13
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationF16A in binding proteinPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Arch-EEQ
Plasmid#45188PurposeArch voltage indicator with double mutations D95Q-D106E (Arch-EEQ), with faster kinetics and greater fluorescence dynamic range than Arch-D95NDepositorInsertenhanced Archaerhodopsin
UseLentiviralTagseYFPExpressionMammalianMutationD95Q, D106EPromoterCamKIIaAvailable sinceJuly 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pML3009
Plasmid#193256PurposeGamma-aminobutyric acid sensor expressing eYFP reporterDepositorInsertsgabR
eYFP
UseSynthetic BiologyTagsExpressionMutationPromotersynthetic promoter P2 and synthetic promoter PGab…Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAL189
Plasmid#22278PurposeExpresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitmentDepositorInsertIntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLBC0060
Plasmid#172783PurposeLevel 0 module containing NT2_cYFP3XHADepositorInsertCDS C-terminal eYFP (175-238) 3XHA tag
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBC0061
Plasmid#172784PurposeLevel 0 module containing CT_cYFP3XHADepositorInsertCDS C-terminal eYFP (175-238) 3XHA tag
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLIPglu-3.2mDelta13
Plasmid#12992DepositorInsertFLIPglu F16A S112A Delta13
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationF16A, S112A in binding proteinPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFLIPglu-170nDelta13
Plasmid#12989DepositorInsertFLIPglu F16F Delta13
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationWT binding protein (no F16A)PromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTEI135
Plasmid#126073PurposeVector for expression of mitochondria targeted YFP1-10 (T203Y) in plant cellDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseBinaryTagsYFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI165
Plasmid#126074PurposeVector for expression of plastid targeted improved YFP1-10 (T65G,T205S, T203Y) in plant cellDepositorInsertChloroplastic FNR transit peptide
UseBinaryTagsYFP1-10 (improved)ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI166
Plasmid#126075PurposeVector for expression of mitochondria targeted improved YFP1-10 (T65G, T205S, T203Y) in plant cellDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseBinaryTagsYFP1-10 (improved)ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLBT0130
Plasmid#172809PurposeLevel 1 module containing 35S-CTP-mCHERRY-cYFP_P3 FDepositorInsertp35S-5UTMV-CTPSSU-mCHERRY-cYFP3XHA-3U+TER35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBT0120
Plasmid#172808PurposeLevel 1 module containing 35S-CTP-mCHERRY-nYFP_P2 FDepositorInsertp35S-5UTMV-CTPSSU-mCHERRY-3XFLAGnYFP-3U+TER35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBT0050
Plasmid#172801PurposeLevel 1 module containing 35S-HSP21-cYFP_P3 FDepositorInsertp35S-5UTMV-CTPSSU-mHSP21-cYFP3XHA-3U+TER35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBT0020
Plasmid#172798PurposeLevel 1 module containing 35S-HSP21-nYFP_notag_P2 FDepositorInsertp35S-5UTMV-CTPSSU-mHSP21-nYFP-3U+TER35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBT0030
Plasmid#172799PurposeLevel 1 module containing 35S-HSP21-cYFP_notag_P3 FDepositorInsertp35S-5UTMV-CTPSSU-mHSP21-cYFP-3U+TER35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only