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Plasmid#217720PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRD1 (ADGRD1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-550PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF5 CTF-FLAG
Plasmid#217698PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF5 (ADGRF5 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-990PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG3 CTF-FLAG
Plasmid#217701PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG3 (ADGRG3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-249PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG1 CTF-FLAG
Plasmid#217699PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG1 (ADGRG1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-382PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF1 CTFdeltaTA-FLAG
Plasmid#217727PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF1 (ADGRF1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-572PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG4 CTFdeltaTA-FLAG
Plasmid#217735PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG4 (ADGRG4 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2727PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG3 CTFdeltaTA-FLAG
Plasmid#217734PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG3 (ADGRG3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-255PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG7 CTFdeltaTA-FLAG
Plasmid#217738PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG7 (ADGRG7 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-421PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-2xflox-BRX-eGFP-NLS
Plasmid#217535PurposeAAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivoDepositorArticleInsert4X BRE reporter and miniXon casette (Smad1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterBMP response element (BRE)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCM2_I432D_eGFP
Plasmid#208879PurposeExpress eGFP-CCM2 with the I432D mutation in mammalian cells.DepositorArticleInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationmutation I432DPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_35-345_eGFP
Plasmid#208881PurposeExpress eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.DepositorArticleInsertTLNRD1 (TLNRD1 Human)
UseTagseGFPExpressionMammalianMutationamino acid sequence 35-345PromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mito-PDCD10-mScarlet-I
Plasmid#208887PurposeExpress mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.DepositorArticleInsertCCM3 (PDCD10 Human)
UseTagsmScarlet-IExpressionMammalianMutationPDCD10 targeted to the mitonchondriaPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_5H_eGFP
Plasmid#208861PurposeExpress eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.DepositorArticleInsertTLNRD1 5 helix bundle (TLNRD1 Human)
UseTagsExpressionMammalianMutationDeleted amino acid 142-273PromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCM2_wt_eGFP
Plasmid#208876PurposeExpress eGFP-CCM2 in mammalian cells.DepositorArticleInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationWT sequencePromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCM2_I428S_eGFP
Plasmid#208878PurposeExpress eGFP-CCM2 with the I428S mutation in mammalian cells.DepositorArticleInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationmutation I428SPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
1114H
Plasmid#200640PurposeAn. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile malesDepositorInsertgRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Zeo-H2BC11
Plasmid#207759PurposeDonor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorArticleInsertH2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRISPomyces-AsCas12j-2
Plasmid#191655PurposeAll-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2DepositorInsertAsCas12j-2
UseTagsExpressionBacterialMutationPromoterrpsL(XC)-BbsIAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1067B
Plasmid#200252PurposepBac-U6-gRNA(doublesex)-3xp3-tdTomatoDepositorInsert4 gRNAs targeting Doublesex
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggybac-EF1-halo-Oct4
Plasmid#197909Purposesingle-particle tracking for OCT4DepositorInsertpou5f1 (Pou5f1 Mouse)
UseTagsHalotagExpressionMammalianMutationPromoterEF1Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only