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Plasmid#198570PurposeSPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vectorDepositorInsertSPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)
UseRetroviralTags3xFlagExpressionMammalianMutationchanged arginine R76 to alanine (R76A)PromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IRES-blast GFP-SPNS1(E164K)
Plasmid#198572PurposeSPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vectorDepositorInsertSPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
UseRetroviralTags3xFlag-GFPExpressionMammalianMutationchanged glutamic acid E164 to lysine (E164K)PromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-muGFP-hATG2A mLIRΔWIR
Plasmid#215508PurposeExpresses GFP tagged human ATG2A which has the mutation in LC3 interacting region and lacks WIPI interacting region.DepositorInsertAutophagy related 2A (ATG2A Human)
UseRetroviralTagsmuGFPExpressionMammalianMutationP656R (natural variant with no functional relevan…PromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-ACSL4-(-1899)
Plasmid#202514PurposeExpressing luciferase driven by human ACSL4 promoter (bases -1 to -1899 with respect to exon1 start site)DepositorInsertACSL4 promoter (partial) (ACSL4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-ACSL4-(-706/-277)
Plasmid#202511PurposeExpressing luciferase driven by human ACSL4 promoter (bases -277 to -706 with respect to exon1 start site)DepositorInsertACSL4 promoter (partial) (ACSL4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-ACSL4-(-254)
Plasmid#202512PurposeExpressing luciferase driven by human ACSL4 promoter (bases -1 to -254 with respect to exon1 start site)DepositorInsertACSL4 promoter (partial) (ACSL4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-ACSL4-(-706)
Plasmid#202513PurposeExpressing luciferase driven by human ACSL4 promoter (bases -1 to -706 with respect to exon1 start site)DepositorInsertACSL4 promoter (partial) (ACSL4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2 RB1 sgRNA #2
Plasmid#202519PurposeExpressing Cas9 and sgRNA targeting human RB1 geneDepositorInsertRB1 sgRNA (RB1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-mADAR2-short
Plasmid#175588Purposemammalian expression of mouse short Adar2, blasticidine selectionDepositorInsertShort Mouse Adar2
UseTagsFlagExpressionMammalianMutationPromotercmvAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-tBid-2E-pEGFP-C1
Plasmid#166747PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation to 2 positions in the BH3 domainDepositorInserttBid-2E (Bid Mouse)
UseTagsVenusExpressionMammalianMutation2 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LRP5_Ldl-recept-b
Plasmid#109867PurposeProtein expression and purification of LRP5_Ldl-recept-bDepositorInsertLRP5_Ldl-recept-b (LRP5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LRP5_Ldl-recept-b
Plasmid#109785PurposeProtein expression and purification of LRP5_Ldl-recept-bDepositorInsertLRP5_Ldl-recept-b (LRP5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX335A_hCas9(D10A)_IRF8gRNA4
Plasmid#89722PurposeKnockout IRF8 in human cellsDepositorInsertIRF8 gRNA4 (IRF8 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335A_hCas9(D10A)_IRF8gRNA3
Plasmid#89721PurposeKnockout IRF8 in human cellsDepositorInsertIRF8 gRNA3 (IRF8 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_hBcl10_#2
Plasmid#75254Purposeretroviral shRNA vector against human BCL10, expresses GFP for transduction controlDepositorInsertshRNA against hBcl10 (BCL10 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_hBcl10_#1
Plasmid#75248Purposeretroviral shRNA vector against human BCL10, expresses GFP for transduction controlDepositorInsertshRNA against hBcl10 (BCL10 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_hMIB2_#9
Plasmid#75255Purposeretroviral shRNA vector against human MIB2, expresses GFP for transduction controlDepositorInsertshRNA against MIB2 (human) (MIB2 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecBCD GamS
Bacterial Strain#176586PurposeE. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.DepositorBacterial ResistanceChloramphenicol and ampicillinAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecB GamS
Bacterial Strain#176585PurposeE. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.DepositorBacterial ResistanceChloramphenicol and ampicillinAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
hTLR2 flag
Plasmid#13082DepositorInsertTLR2 (TLR2 Human)
UseTagsFlagExpressionMammalianMutationAmino acids 1-16 have been replaced with mammalia…PromoterAvailable sinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only