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Plasmid#63187PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only
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pAV116.loxP
Plasmid#63188PurposepRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y
Plasmid#60393PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)DepositorInsertTUB1 (TUB1 Budding Yeast, Synthetic)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ypq2-2GFP-URA
Plasmid#207010PurposeIntegration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertYPQ2
UseTags2xGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Vph1-2GFP-URA
Plasmid#207009PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertVPH1
UseTags2xGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
p426-Cup1p-FusionRed
Plasmid#188392PurposeCu2+-dependent expression of red fluorescent protein in yeast cellsDepositorInsertFusionRed
UseTagsExpressionYeastMutationPromoterCUP1 promoterAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
p21StBD
Plasmid#190269PurposeConstitutively expresses a protein of interest fused to a GAL4 DNA-binding domain. Targeted integration to genomic locus YPRC-tau 3, Chromosome XVI.DepositorTypeEmpty backboneUseSynthetic Biology; Yeast genome-integrating vectorTagsGAL4-BDExpressionMutationPromoterTDH3Available sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 + VP2C-MIOX
Plasmid#166680PurposeYeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-MIOX
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)
Plasmid#166854PurposeExpresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeastDepositorInsertAtg13[571–700] (ATG13 Budding Yeast)
UseTags6xHisExpressionYeastMutationF641E, I645EPromoterpCuAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)
Plasmid#166853PurposeExpresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeastDepositorInsertAtg13[571–700] (ATG13 Budding Yeast)
UseTags6xHisExpressionYeastMutationF641G, I645GPromoterpCuAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK_lacO-cP-AOX1-AtX+NpgA
Plasmid#126727Purposeyeast plasmid overexpressing AtX and NpgADepositorInsertatX,npgA
UseTags6xHisExpressionYeastMutationPromoterAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2
Plasmid#126734Purposeyeast plasmid overexpressing ADH2 from Saccharomyces cerevisiaeDepositorInsertadh2
UseTags6xHisExpressionYeastMutationPromoterGAPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_-P-GAP-NpgA+AtX
Plasmid#126725Purposeyeast plasmid overexpressing AtX and NpgADepositorInsertatX,npgA
UseTags6xHisExpressionYeastMutationPromoterGAPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-LovC
Plasmid#126729Purposeyeast plasmid overexpressing LovCDepositorInsertlovC
UseTags6xHisExpressionYeastMutationPromoterAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-LovB
Plasmid#126728Purposeyeast plasmid overexpressing LovBDepositorInsertlovB
UseTags6xHisExpressionYeastMutationT7227A,T8994APromoterAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]
Plasmid#106469PurposeExpresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(S219,D415,H474A)
UseTagsGFPExpressionBacterial and YeastMutationChanged Serine 219, Aspartate 415 and Histidine 4…PromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB3 Cterminal tail delta K
Plasmid#60404PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPKDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K3.loxP
Plasmid#63202PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5.loxP
Plasmid#63204PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO3.loxP
Plasmid#63205PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only