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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3
Plasmid#98974PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 3 and Cas9(D10A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCVL.Active/Repressed TLR (Sce)
Plasmid#45575PurposeCodes for the Active/Repressed TLR with the target site for I-Sce I nucleaseDepositorInsertseGFP with I-Sce I TS
+3 mCherry
UseLentiviralTagsT2A dislinker and iRFPExpressionBacterial and MammalianMutationembedded I-Sce I TS from 163-185PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
p21 shRNA1
Plasmid#25868DepositorInsertp21 (Cdkn1a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs ABCDE>A
Plasmid#83318Purposehuman DNA-PKcs/ABCDE>alaDepositorInserthuman DNA-PKcs (PRKDC Human)
UseTagsExpressionMammalianMutation6 phosphorylation sites in the ABCDE cluster subs…PromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
7xE-Box::Renilla
Plasmid#124532Purposec-Myc Renilla luciferase reporterDepositorInsertc-Myc Renilla reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
7xSMAD_RE::FLuc
Plasmid#124531PurposeSMAD2 FLuc luciferase reporterDepositorInsertSMAD FLuc reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET41-MBP-t-A3A-t-His
Plasmid#109231PurposeBacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV proteaseDepositorInserthuman APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MEK5DD-HA
Plasmid#65247PurposeExpression of HA tagged dominant active MEK5 in mammalian cellsDepositorInsertMEK5DD (MAP2K5 Human)
UseTagsHAExpressionMammalianMutationchanged Ser311 to Asp, Ser315 to AspPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:ELuc
Plasmid#118062PurposeTranscriptional unit for constitutive expression of ELuc in mammalian cellsDepositorInsertCMV:ELuc:bGH
UseLuciferase and Synthetic Biology; Assembly of gb2…TagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro-NFLAG TRF1 deltaA deltaM
Plasmid#16297DepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationDeleted acidic domain (aa1-66) and myb domain (aa…PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5 CASTOR1-Flag Cterm
Plasmid#84488Purposeoverexpression of CASTOR1 with Flag tagDepositorInsertCASTOR1 (GATSL3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
CABE-T3.1
Plasmid#193267PurposeExpression of CABE-T3.1 base editor in mammalian cells, CMV promoterDepositorInsertCABE-T3.1
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Flag CASTOR1 I280A
Plasmid#84495Purposeoverexpression of CASTOR1 I280A mutantDepositorInsertCASTOR1 I280A (GATSL3 Human)
UseTagsFLAGExpressionMammalianMutationI280APromoterCMVAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRi(v1)-Blast
Plasmid#170067PurposeExpresses dCas9 repressor KRAB-dCas9 and blasticidin resistanceDepositorInsertKRAB-dCas9
UseCRISPR and LentiviralTags2A and 2xHA tagExpressionMammalianMutationPromoterEFS-NSAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)
Plasmid#159660PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)
Plasmid#159661PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)
Plasmid#159659PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRedF
Plasmid#118057PurposeGBPart - RedF Luciferase CDSDepositorInsertRed Firefly Luciferase CDS
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationRed mutant S286YPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only
