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Plasmid#191681PurposeDonor plasmid backbone PGK-neoRDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only
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AAV-SYN_mCyRFP2-CREB
Plasmid#137008PurposeAAV backbone, mCyRFP2-CREB donor for R-CREBDepositorInsertCREB1 (Creb1 Rat)
UseAAVTagsmCyRFP2ExpressionMammalianMutationPromoterhSynapsinAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYL236
Plasmid#173184PurposeCRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 (EZH2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-EF1a-mTagRFP-T-CAAX
Plasmid#167973PurposeAAVS1 targeting repair templateDepositorUseCRISPR; Donor templateTagsmTagRFP-T-CAAXExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bxb1_AJMA
Plasmid#183760PurposeBxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703DepositorInsertsASAP2f
jRCaMP1b
miRFP703
ASAP2f
UseTagsLck and NESExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
FOR human NANOS3 TALEN
Plasmid#59722PurposeTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)DepositorInsertHD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
UseTALENTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
REV human NANOS3 TALEN
Plasmid#59723PurposeTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)DepositorInsertNG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
UseTALENTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
TV-hPITX3-forward
Plasmid#22073DepositorInsertPITX3 (ASMD Human)
UseCre/Lox; Zfn donorTagseGFPExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN_mEGFP-CREB
Plasmid#137006PurposeAAV backbone, mEGFP-CREB donor for G-CREBDepositorInsertCREB1 (Creb1 Rat)
UseAAVTagsmEGFPExpressionMammalianMutationPromoterhSynapsinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
HES7-NLuc-2A-tdTomato
Plasmid#130932PurposeDonor vector for tagging human HES7 C-terminus with NanoLuc and tdTomatoDepositorInsertHES7 (HES7 Human)
UseTagsNanoLuc and tdTomatoExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-CREB (S133A)
Plasmid#137002PurposemEGFP-CREB donor for G-CREB with S133A mutationDepositorInsertCREB1 (Creb1 Rat)
UseTagsmEGFPExpressionMammalianMutationChanges Serine 133 into AlaninePromoterCMVAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
SOX2 KO (T2A-H2B-EGFP)
Plasmid#167969PurposeSOX2 CDS replacement with T2A-H2B-EGFPDepositorInsertSRY-Box Transcription Factor 2 (SOX2 Human)
UseCRISPR; Donor templateTagsH2B-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-NanoLuc-Puromycin
Plasmid#167209PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsNanoLucExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR-U6gRNA-PGKpuro2ABFP
Plasmid#136405PurposeDonor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationBbsI restriction site at position 437 was removed…PromoterU6, PDKAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_KMT2B
Plasmid#101070PurposeDonor vector for 3' FLAG tag of human KMT2BDepositorInsertKMT2B homology arms (KMT2B Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX4
Plasmid#183903Purposedonor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locusDepositorInsertGFP
UseTagsExpressionInsectMutationPromoterAedes aegypti PUbAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX3
Plasmid#183904Purposedonor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locusDepositorInsertGFP
UseTagsExpressionInsectMutationPromoterAedes aegypti PolyubiquitinAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry
Plasmid#179428PurposeDual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertTRE-cit-(nospacer)-mch
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-CNL
Plasmid#65712PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-CNL)DepositorInsert7xTcf, minCMV, 3xNLS, CNL
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-ONL
Plasmid#65713PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-ONL)DepositorInsert7xTcf, minCMV, 3xNLS, ONL
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only