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Showing: 2461 - 2480 of 3246 results

  2. pRDA_122

    Plasmid
    #208095
    Purpose
    Perturb-Seq lentiviral gRNA expression vector with a 3' appended capture sequence to enable direct capture of CRISPR gRNAs for scRNA-seq
    Depositor
    John Doench
    Article
    Geiger-Schuller et al bioRxiv 2023.01.23.525198
    Insert
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    208095-plasmid-map-sequence-id-405878

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  7. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

    Plasmid
    #216732
    Purpose
    Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG lentiviral guide RNA with eGFP tag
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Availability
    Academic Institutions and Nonprofits only
    216732-plasmid-map-sequence-id-430316

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  8. pAAV-U6-sgCTG-CMV-GFP

    Plasmid
    #216734
    Purpose
    AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and CMV
    Availability
    Academic Institutions and Nonprofits only
    216734-plasmid-map-sequence-id-430319

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  9. pAAV-nEF-SpCas9D10A nickase

    Plasmid
    #216736
    Purpose
    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    EF-1-alpha core
    Availability
    Academic Institutions and Nonprofits only
    216736-plasmid-map-sequence-id-430323

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  10. pAAV-CMV-SpCas9D10A nickase

    Plasmid
    #216737
    Purpose
    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMVd1
    Availability
    Academic Institutions and Nonprofits only
    216737-plasmid-map-sequence-id-430337

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  11. His6-TEV-CRBN-midi

    Plasmid
    #215330
    Purpose
    CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design
    Depositor
    Alessio Ciulli
    Article
    Kroupova et al bioRxiv 2024.01.17.575503
    Insert
    CRBN (CRBN Human)
    Use
    Tags
    6x HIS and TEV
    Expression
    Bacterial
    Mutation
    C78I, I92V, K116N, Q134E, R283W, C287N, V293S, G3…
    Promoter
    lacI
    Availability
    Academic Institutions and Nonprofits only
    215330-plasmid-map-sequence-id-426717

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  12. pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag

    Plasmid
    #210820
    Purpose
    Mammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)
    Depositor
    Luke Chao
    Article
    Bell et al bioRxiv 2023.11.08.566258
    Inserts
    Prominin 1 (PROM1 Human)
    Protocadherin 21 (CDHR1 Human)
    Use
    Tags
    Myc, 3xFlag and TwinStrep, 10xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and CMV / IRES2
    Availability
    Academic Institutions and Nonprofits only
    210820-plasmid-map-sequence-id-417732

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  16. M13cp-dg1 hp

    Plasmid
    #218093
    Purpose
    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pI
    Depositor
    Kevin Esvelt
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    218093-plasmid-map-sequence-id-432465

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  17. M13cp-dg2 hp

    Plasmid
    #218094
    Purpose
    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pII
    Depositor
    Kevin Esvelt
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    218094-plasmid-map-sequence-id-437754

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  18. M13cp-dg3 hp

    Plasmid
    #218095
    Purpose
    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII
    Depositor
    Kevin Esvelt
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    218095-plasmid-map-sequence-id-432463

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  19. LacI pm

    Plasmid
    #218097
    Purpose
    Phagemid that expresses LacI but no phage proteins, used as a control
    Depositor
    Kevin Esvelt
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    218097-plasmid-map-sequence-id-432441

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Showing: 2461 - 2480 of 3246 results