Addgene

We narrowed to 14,717 results for: LEA

Showing: 2461 - 2480 of 14717 results

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pNIC-HIS6-GST-TEV-AVITAG-GG

Plasmid

#215809

Purpose

Cloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEM7074

Plasmid

#214012

Purpose

PrpsM driven expression of ssrA-tagged mCerulean (ASV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCerulean-ASV

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSYC-187

Plasmid

#74794

Purpose

pCS4+-P2A-mCherry-CAAX

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

P2A-mCherry-CAAX

Use

Tags

Expression

Mutation

Promoter

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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Reb_82 aka PAS623

Plasmid

#71228

Purpose

Reb_1 with RebB(S82P, D88G)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Use

Tags

none

Expression

Bacterial

Mutation

RebB(S82P, D88G), RebA (G53A)

Promoter

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pTXB1-AbaSFI

Plasmid

#86900

Purpose

Express CBD and intein tag containing AbaSI enzyme full length

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

AbaSDFI

Use

Tags

CBD

Expression

Mutation

Promoter

T7

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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2xmNeonGreen-hMeikin

Plasmid

#174704

Purpose

Stable expression of human Meikin

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Use

Retroviral

Tags

2xmNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP-HBx NESM

Plasmid

#24932

Depositor

Xin Wang

Article

Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

Insert

HBx

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

L98A,G99A,L100A

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pET28a-Hisx6-Sirt6

Plasmid

#20277

Depositor

Leonard Guarente

Article

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
DinoIII-arrO

Plasmid

#128142

Purpose

Dinoflagellate backbone for rifampin resistant codon optimized gene

Depositor

Senjie Lin

Article

Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

Insert

Rifampin ADP-ribosylating transferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Dinolagellate potential promoters

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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p2CT-Cdi

Plasmid

#83044

Purpose

Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

Depositor

Jennifer Doudna

Article

Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

Insert

CdiCas9

Use

Tags

His-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only
pCS2TAL3-DDD

Plasmid

#48637

Purpose

Expression of TALEN proteins with FokI-DDD domain

Depositor

David Grunwald

Article

Hu et al PLoS One. 2013 May 7;8(5):e63074. doi: 1

Type

Empty backbone

Use

TALEN

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-RRR

Plasmid

#48636

Purpose

Expression of TALEN proteins with FokI-RRR domain

Depositor

David Grunwald

Article

Hu et al PLoS One. 2013 May 7;8(5):e63074. doi: 1

Type

Empty backbone

Use

TALEN

Tags

HA-1 and HA-2

Expression

Mutation

Promoter

CMV

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pXcX-PRS-EGFP

Plasmid

#25865

Depositor

Sergey Kasparov

Article

Teschemacher et al Hypertension. 2008 Aug . 52(2):351-8.

Insert

PRS driven enhanced green fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2010

Availability

Academic Institutions and Nonprofits only

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P100 (3OMC)

Plasmid

#28159

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SND1 (SND1 Human)

Use

Tags

His tag with TEV cleavage

Expression

Bacterial

Mutation

Tudor domain (residues 650-910).

Promoter

Available since

Feb. 28, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pET21b-RL018A

Plasmid

#42137

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mCerulean-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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pT7RNAP-Q737P

Plasmid

#175356

Purpose

Support plasmid for T7_ori plasmids

Depositor

Sven Panke

Article

Becker et al Nucleic Acids Res. 2021 Jul 13. pii: 632

Insert

T7RNAP-Q737P

Use

Tags

Expression

Bacterial

Mutation

Q737P

Promoter

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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No-repeats

Plasmid

#122487

Purpose

A control plasmid that is similar to PRExpress but lacks poly-PRE sequence. (GenBank: MF058599.1)

Depositor

Renato Paro

Article

Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

Insert

LacZ

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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LIP-WT

Plasmid

#16657

Depositor

Bert Vogelstein

Article

Cheong et al Science. 2006 Nov 24. 314(5803):1308-11.

Insert

Liposomase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pPS2045

Plasmid

#1376

Depositor

Pamela Silver

Article

Brodsky et al RNA 2000 Dec;6(12):1737-49.

Insert

U1A (1-94) (RNU1-4 Human)

Use

Tags

GFP

Expression

Yeast

Mutation

U1A (1-94)

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pISH-OCAM

Plasmid

#15932

Depositor

Peter Mombaerts

Article

Li et al Nature. 2004 Mar 25. 428(6981):393-9.

Insert

OCAM (Ncam2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only