We narrowed to 16,103 results for: sgRNA
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Plasmid#155108Purposelentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZDepositorInsertLacZ_sgRNA_2
UseCRISPR and LentiviralExpressionMammalianPromoterU6 promoterAvailable SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
HCP1
Plasmid#166103PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.DepositorAvailable SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP2
Plasmid#166104PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorAvailable SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP3
Plasmid#166105PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CASI-InteinC-SpRY C aa714-1368-U6-Camk2d sgRNA
Plasmid#209788PurposeExpresses SpRY cas9C by the constitutive CASI promoter and sgRNA targeting the oxidative activation sites of Camk2d by U6 promoterDepositorInsertSpRY C, Camk2d sgRNA
UseAAVPromoterCASIAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M7NS_AmpR
Plasmid#119157PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
UseCrisprPromoterJ23119Available SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pV1200
Plasmid#111429PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM710
Plasmid#68893PurposeIPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJMP1346
Plasmid#119274Purposeknockdown folA in K. pneumoniaeDepositorInsertsgRNA folA (K. pneumoniae)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1221
Plasmid#119260Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9
Plasmid#87917PurposeGateway cloningDepositorInsertsgHTT1, sgGFP2, shCas9
ExpressionMammalianPromoterH1, U6, 7SKAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM705
Plasmid#68892PurposeIPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
Available SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA AAVS1
Plasmid#128119PurposeCo-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA AAVS1 + GFP
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterRSV/U6Available SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-B_2xsgRNAs_TRNAU1AP (pAVA3291)
Plasmid#254883PurposeLentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting TRNAU1APDepositorInsertTwo sgRNAs targeting TRNAU1AP
UseCRISPR and LentiviralPromoterU6/7SKAvailable SinceJune 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-B_2xsgRNAs_PRPF39 (pAVA3245)
Plasmid#254885PurposeLentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting PRPF39DepositorInsertTwo sgRNAs targeting PRPF39
UseCRISPR and LentiviralPromoterU6/7SKAvailable SinceJune 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCF820-sgNT-1_U6-sgRNA-EF1a-mCherry2
Plasmid#211648PurposeU6-sgRNA-EF1a-mCherry2DepositorInsertNon-targeting control sgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sox3gRNA-T2
Plasmid#127778PurposeChicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)DepositorInsertsox3gRNAT2
UseCRISPRAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
gRNA telo/GFP
Plasmid#137713PurposeVector co-expressing sgRNA telo and GFPDepositorInsertsgRNA telo + GFP
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianPromoterU6/RSVAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-sgRNA-MS2-U6
Plasmid#171694PurposeTo construct expression vector for tBE-V5-mA3DepositorInsertsgRNA scaffold-MS2-U6
UseCRISPRExpressionMammalianAvailable SinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-2x
Plasmid#164414PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only