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We narrowed to 9,093 results for: sgRNA

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Showing: 2441 - 2460 of 9093 results

  3. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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  6. lentiCRISPR - Amplicon, JAK2 sgRNA 2

    Plasmid
    #70660
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

    Plasmid
    #188902
    Purpose
    dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    dCas9-KRAB(Kox1)-MeCP2
    Use
    Lentiviral
    Tags
    NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…
    Promoter
    SFFV
    Available Since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188902-plasmid-map-sequence-id-372712

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  15. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid
    #136659
    Purpose
    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136659-plasmid-map-sequence-id-273359

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  18. pLenti-AncBE4max

    Plasmid
    #174164
    Purpose
    This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.
    Depositor
    Wensheng Wei
    Article
    Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103
    Insert
    AncBE4max
    Use
    Lentiviral
    Tags
    P2A-mCherry
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174164-plasmid-map-sequence-id-342437

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Showing: 2441 - 2460 of 9093 results