We narrowed to 8,708 results for: sgRNA

1. pX330-PITCh-TUBA1B

   Plasmid

   #207763

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAS1yl

   Plasmid

   #73226

   Purpose

   Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells

   Depositor

   Sheng Yang

   Article

   Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR; In y. lipolytica

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   unknown

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS614_pTetR-P2A-BFPnls/sgNS

   Plasmid

   #108650

   Purpose

   Non-specific Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Non-specific sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKRG3-mU6-PUb-hSpCas9

   Plasmid

   #162162

   Purpose

   Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   N/A

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLC-mCherry-MLKL

   Plasmid

   #75167

   Purpose

   LentiCRISPR-mCherry with sgRNA targeting human MLKL

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Insert

   MLKL sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-CETN2

   Plasmid

   #227291

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of CETN2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of CETN2 (CETN2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLGR002 LGR Cas9 guide vector

   Plasmid

   #188320

   Purpose

   Universal backbone for CRISPR/Cas9 sgRNA expression

   Depositor

   Laralynne Przybyla

   Article

   Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   PuroR-T2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   pU6 5'CMV and EF1-alpha

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-CENPA

   Plasmid

   #227282

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of CENPA (CENPA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3015b

   Plasmid

   #209317

   Purpose

   Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

   Depositor

   Thomas Hooven

   Article

   Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

   Insert

   Group B Streptococcus-compatible single guide RNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Xyl/tet promoter that is constitutively active

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLC-EGFP-RIP3

    Plasmid

    #75165

    Purpose

    LentiCRISPR-EGFP with sgRNA targeting human RIP3

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    RIP3 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180266

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    No-Target Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS613_pTetR-P2A-BFPnls/sgTelo

    Plasmid

    #108649

    Purpose

    Telomere-targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Telomere-targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXPR_053

    Plasmid

    #113591

    Purpose

    Constitutive expression of a single sgRNA and the fluorophore violet-excited GFP in immune cells

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Commun. 2019 Apr 10;10(1):1668. doi:

    Insert

    sgRNA cloning site
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. LRG2.1_Neo

    Plasmid

    #125593

    Purpose

    Lentiviral expression of sgRNA with GFP and neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Biomolecular FeedBack (FB)

    Plasmid

    #109391

    Purpose

    This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pJ23100mut-dCas9-T- phtpG1-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57-Amp_CRISPIE donor B2

    Plasmid

    #172843

    Purpose

    CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mRuby3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEPQD0KN0750

    Plasmid

    #185627

    Purpose

    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    lacZ
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX335-EN475

    Plasmid

    #86231

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX335-EN477

    Plasmid

    #86232

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHDR-USR-eGFP

    Plasmid

    #208830

    Purpose

    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.

    Depositor

    Zhiying Zhang

    Article

    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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