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Plasmid#44011PurposeLentiviral shRNA vector (pGIPz based) for inducible knockdownDepositorInsertPheS Gly294
UseLentiviralTagsRFPExpressionMammalianMutationPromoterTRE2Available sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-CAG-F14F15S-Switch
Plasmid#99733PurposeFlp-based FLEX reporter (from RFP to GFP)DepositorInsertTagRFP and EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF3428
Plasmid#144904PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTRIM27 (TRIM27 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMVA2
Plasmid#119951PurposeExpresses recombinant MVA pathway in Escherichia coliDepositorInsertsmvaE
mvaS
mvaK1
mvaK2
mvaD
idi
UseTagsExpressionBacterialMutationA110GPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5xQUAS-ZsGreen-P2A, α-cry:mCherry
Plasmid#180477PurposeInducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherryDepositorInserts5x QUAS-ZsGreen-P2A
mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-UBC9-sh1
Plasmid#168986PurposeExpresses RFP cDNA and UBC9 shRNA 1 in mammalian cellsDepositorInsertUbiquitin Conjugating Enzyme 9 (UBE2I )
UseTagsExpressionMammalianMutationPromoterUbc promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDD284
Plasmid#66825PurposeTagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized TagRFP-TExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIM700
Plasmid#74639Purposealias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBD21_037
Plasmid#109154PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720DepositorInsertsmRFP
iRFP720
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIM690
Plasmid#74638Purposealias pFA6a-yTagRFP-T-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and hygromycin selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-15
Plasmid#119994PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIM572
Plasmid#74640Purposealias pFA6a-yTagRFP-T-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagRFP-T and G418 selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb713-HO
Plasmid#141199PurposeExpresses mRhubarb713 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb713
heme oxygenase
UseTags6x-HisExpressionBacterialMutationR134H, W309R, Q310A compared to iRFPPromoterT7Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb720-HO
Plasmid#141201PurposeExpresses mRhubarb720 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb720
heme oxygenase
UseTags6x-HisExpressionBacterialMutationR134H, L196Q, T202D, V203I, W309R, Q310A compared…PromoterT7Available sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z15
Plasmid#120058PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb719-HO
Plasmid#141200PurposeExpresses mRhubarb719 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb719
heme oxygenase
UseTags6x-HisExpressionBacterialMutationR134H, Y198S, T202Y, V203I, W309R, Q310A compared…PromoterT7Available sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P15
Plasmid#120026PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
ptfLC3
Plasmid#21074PurposeMammalian expression of rat LC3 fused to mRFP and EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsEGFP and mRFPExpressionMammalianMutationPromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dInPAkt
Plasmid#181834PurposeGenetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).DepositorInsertLyn-dInPAkt
UseTagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC17
Plasmid#118875Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal tagRFPDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-tagRFP-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and tagRFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only