Addgene

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GFP KNL1 wt hardened

Plasmid

#45223

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

GFP KNL1 wt hardened (mutated KNL1 full length that is resisted to KNL1 siRNA) (KNL1 Human)

Use

Tags

TEV S15

Expression

Mutation

E172G

Promoter

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pEF1a-nSpCas9-NG

Plasmid

#171692

Purpose

Expresses nSpCas9-NG in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

nSpCas9-NG

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…

Promoter

EF1a

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse c-MycV394D-IRES-RFP

Plasmid

#73573

Purpose

Mammalian retroviral expression of mouse Myc V394D mutant

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

cMyc (Myc Mouse)

Use

Retroviral

Tags

IRES-RFP

Expression

Mammalian

Mutation

V394D

Promoter

MSCV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
J-sRNA-331Bb

Plasmid

#78285

Purpose

An inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase mRNA

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

j-sRNA-331

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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p7135 pHAGE-P-CMVt-N-HA-GAW-EIF3E

Plasmid

#100147

Purpose

Expresses EIF3E

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

EIF3E (EIF3E Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Beclin-BATS

Plasmid

#30498

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 Apr 25. (

Insert

Beclin, Barkor (BECN1, ATG14 Human)

Use

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

Beclin 1 + 413-492 aa of Barkor

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
AdMKP-3 S197A

Plasmid

#131522

Purpose

Expresses MKP-3 with S197A mutation in mammalian cells

Depositor

Haiyan Xu

Article

Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

Insert

Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Expression

Mutation

changed Serine 197 to Alanine

Promoter

CMV

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-cMyc

Plasmid

#136614

Purpose

Dox-inducible lentiviral vector expressing mouse cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

cMyc (Myc Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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TFORF3502

Plasmid

#144978

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

VENTX (VENTX Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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mito-yHS1-M7A

Plasmid

#159164

Purpose

Mitochondrial labile heme reporter

Depositor

Amit Reddi

Article

Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

Insert

mito-yHS1-M7A

Use

Tags

COX4 pre-sequence

Expression

Yeast

Mutation

Mutated Met 7 of the cyt b562 module of mito-yHS1…

Promoter

GPD

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-SasgRNA scaffold-CMV-nSaCas9

Plasmid

#171695

Purpose

Expresses nSaCas9 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

sgRNA scaffold-nSaCas9-NLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A

Promoter

U6 and CMV

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ165

Plasmid

#109327

Purpose

Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoter

Depositor

Yiping Qi

Article

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Insert

zCas9

Use

CRISPR; Gateway compatible zcas9 entry clone

Tags

3x FLAG, NLS and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Promoter

Promoter EC1.2 enhancer fused to EC1.1 promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-KLF3

Plasmid

#49100

Purpose

Expresses KLF3 in mammalian cells

Depositor

Merlin Crossley

Article

Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

Insert

Krüppel-Like Factor 3(KLF3)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only
pENN.AAV.CB7.CI.CNDP2-flag.WPRE.rBG

Plasmid

#132685

Purpose

AAV plasmid encoding mouse CNDP2 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Carnosine dipeptidase 2 (Cndp2 Mouse)

Use

AAV

Tags

Flag

Expression

Mutation

Promoter

Chicken b-actin

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pIS1-Vim/Actb5UTR-renilla

Plasmid

#38238

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Vim promoter with Actb 5UTR/Renilla (Vim, Actb Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Actb promoter (1000 nt upstream) and 5' UTR …

Promoter

Endogenous Vim

Available since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-CERT-PH

Plasmid

#49572

Purpose

expresses the plekstrin homology domain of CERT/OSBP in mammalian cells

Depositor

Marci Scidmore

Article

Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

Insert

GPBP (CERT1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

expresses AA 1-122

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pRS405 PEX14-VAC8-RFP

Plasmid

#166844

Purpose

Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, PEX14 Budding Yeast)

Use

Tags

PEX14 transmembrane domain and RFP

Expression

Yeast

Mutation

PEX14 transmembrane domain

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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hsDCX-EGFP-pHAT

Plasmid

#83918

Purpose

human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II

Depositor

Gary Brouhard

Article

Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

Insert

Doublecortin isoform b (DCX NM_178152.2, Human)

Use

Tags

6-His and EGFP+Strep-tag II

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 8, 2016

Availability

Academic Institutions and Nonprofits only

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mEmerald-Actin-C-18

Plasmid

#53978

Purpose

Localization: Actin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Actin (ACTB Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pRK5 Fyn deltaSH3

Plasmid

#16034

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Use

Tags

Expression

Mammalian

Mutation

Delta SH3: deletion of amino acids 76-141

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only