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Plasmid#169338PurposeExpression of the LT-Fucci(CA) in mammalian cellsDepositorUseTagsHaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only
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pGP-CMV-jGCaMP7f
Plasmid#104483PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7f
UseTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWZL Blast Snail ER
Plasmid#18798DepositorInsertsnail (SNAI1 Human)
UseRetroviralTagsER hormone binding domainExpressionMammalianMutationG525R mutation in the ER. The presence of the G52…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pWZL Blast Twist ER
Plasmid#18799DepositorInserttwist (Twist1 Mouse)
UseRetroviralTagsER hormone binding domainExpressionMammalianMutationG525R mutation in the ER. The presence of the G52…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry
Plasmid#128327PurposeReporter to evaluate YAP1/TEAD-mediated gene transcriptionDepositorInsertsUseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12
Plasmid#101285PurposeLentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFPDepositorInsertE-Cadherin-GFP-H-Ras
UseLentiviralTagsGFPExpressionMammalianMutationH-Ras V12PromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B-E2F1
Plasmid#202520PurposeExpressing human E2F1 protein with FLAG-tagDepositorInsertE2F1 (E2F1 Human)
UseTagsFLAG-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.eC
Plasmid#201903Purposenon-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.eC VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SV40-Galpha-s ONE-GO
Plasmid#204327PurposeGPCR/G protein BRET biosensorDepositorInsertSV40 Galpha-s ONE-GO (GNAS Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-ACSL4-FL
Plasmid#202515PurposeExpressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)DepositorInsertACSL4 promoter (partial) (ACSL4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9-Venus
Plasmid#70267PurposeExpresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.DepositorInsertsCas9
Venus Reporter
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS-NS and P2AAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-mCherry-Halo-YAP1
Plasmid#128336PurposeRetrovirus construct to express mCherry-Halo-YAP1DepositorInsertYAP1 (YAP1 Human)
UseRetroviralTagsmCherry and Halo-tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro
Plasmid#132773PurposeExpression of human LMNA.DepositorInsertLamin A (LMNA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2 RB1 sgRNA #1
Plasmid#202516PurposeExpressing Cas9 and sgRNA targeting human RB1 geneDepositorInsertRB1 sgRNA (RB1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-muscle Amphiphysin 2
Plasmid#22213DepositorInsertAmphiphysin II (BIN1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_FKBP25-V5-APEX2
Plasmid#83418PurposeExpressed FKBP25/FKBP3-V5-APEX2 in mammalian cellDepositorInsertPeptidyl-prolyl cis-trans isomerase FKBP3 (FKBP3 Human)
UseTagsAPEX2 and V5ExpressionMammalianMutationPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335A_hCas9(D10A)_IRF8gRNA1
Plasmid#89719PurposeKnockout IRF8 in human cellsDepositorInsertIRF8 gRNA1 (IRF8 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
cyclin D1-HA-pBabe-Hygro
Plasmid#22703DepositorInsertcyclin D1 (CCND1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMCSF-R(mB)-luc
Plasmid#12422DepositorInsertM-CSF receptor promoter fragment (CSF1R Human)
UseLuciferaseTagsExpressionMutationAML1 binding site mutation (created a KpnI site i…PromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX335A_hCas9(D10A)_IRF8gRNA2
Plasmid#89720PurposeKnockout IRF8 in human cellsDepositorInsertIRF8 gRNA2 (IRF8 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only