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Plasmid#180481PurposeInducible gene expression vector p13xQUAS-MCS, α-cry:mCherryDepositorInsertMultiple cloning site
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse aB crystallin 1.5kb promoter/AcGFP
Plasmid#98104PurposeMouse aB crystallin 1.5kb promoter segment driving GFP expressionDepositorInsertAlpha B-crystallin promoter 1.5 kb fragment, Mus musculus (Cryab Mouse)
UseGfp expressingTagsGFPExpressionMutationPromoterAlpha B-crystallin 1.5 kb promoter fragment (-150…Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PDI-QUAD-V5-pcDNA3.1
Plasmid#153550PurposePDI-QUAD was generated from PDI-WT pcDNA3.1 (+) using two native active sites (CGHC, CGHC) tagged with V5, and mutated (to SGHS, SGHS) using site directed mutagenesis.DepositorInsertProtein disulphide isomerase
UseTagsV5 tagExpressionMutationC53S, C56S, C397S, C400SPromoterpCMVAvailable sinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQFDBD-2x AD*-VP16*, α-cry:EGFP
Plasmid#180473PurposeGene expression vector pQFDBD-2x AD*-VP16*, α-cry:EGFPDepositorInsertQFDBD-2xQFAD*-VP16*
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDest-1.7kbfabp6-eGFP-pA-CrymCherry
Plasmid#159088PurposeLR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA insertsDepositorInsertsUseFluorescent reporterTagsExpressionMutationPromoter1.7kb fabp6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-GGGS-TDP-43
Plasmid#162611PurposeAllows for transcription of mVenus fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-I152L-GGGS-TDP43 M337V
Plasmid#162615PurposeAllows for transcription of improved mVenus I152L fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationM337VPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT2-4xUAS:P2A-mCerulean3
Plasmid#127592PurposeThis vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.DepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mCerulean 156-239-GGGS-TDP-43 M337V
Plasmid#162618PurposeAllows for transcription of mCerulean fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationM337VPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSYC-176
Plasmid#178961PurposeExpresses human GFAP fused to 3xFLAG tag in mammalian cellsDepositorInsertGlial fibrillary acidic protein (GFAP Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMV IE94 PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
p9xQUAS-ZsGreen-P2A, α-cry:mCherry
Plasmid#180479PurposeInducible gene expression vector p9xQUAS-ZsGreen-P2A, α-cry:mCherryDepositorInsert9x QUAS-ZsGreen-P2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse aB crystallin 250bp promoter/AcGFP
Plasmid#98102PurposeMouse aB crystallin 250bp promoter segment driving GFP expressionDepositorInsertAlpha B-crystallin promoter 250 bp fragment, Mus musculus (Cryab Mouse)
UseGfp expressingTagsGFPExpressionMutationPromoterAlpha B-crystallin promoter 250 bp fragment (-259…Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2-CIB1-mCherry-Rab8A
Plasmid#194333PurposeFor use in light-induced Rab8a protein inactivation through the interaction with CRY2DepositorUseTagsmcherryExpressionMammalianMutationPromoterCMV IE94Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
p17xQUAS-Luciferase, α-cry:mCherry
Plasmid#180482PurposeInducible gene expression vector p17xQUAS-Luciferase, α-cry:mCherryDepositorInsertsLuciferase
17x QUAS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse aB crystallin 800bp promoter/AcGFP
Plasmid#98103PurposeMouse aB crystallin 800bp promoter segment driving GFP expressionDepositorInsertAlpha B-crystallin promoter 800 bp fragment, Mus musculus (Cryab Mouse)
UseGfp expressingTagsGFPExpressionMutationPromoterAlpha B-crystallin promoter 800 bp fragment (-833…Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
Anti-PSD [N286/74R]
Plasmid#164159PurposeMammalian Expression Plasmid of anti-PSD (Zebrafish). Derived from hybridoma N286/74.DepositorInsertanti-PSD (Danio rerio) recombinant mouse monoclonal antibody (dlg2 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-CIB1-mRuby-Rab35
Plasmid#194334PurposeFor use in light-induced Rab35 protein inactivation through the interaction with CRY2DepositorUseTagsmRubyExpressionMammalianMutationPromoterCMV IE94Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSmChnERT(#99)
Plasmid#184063Purposecyclofen-inducible mCherry nuclear relocation via mammalian cell transfection or mRNA synthesisDepositorInsertmCherry-nls-ERT2
UseSynthetic BiologyTagsExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5xQUAS-ZsGreen-P2A, α-cry:mCherry
Plasmid#180477PurposeInducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherryDepositorInserts5x QUAS-ZsGreen-P2A
mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-190
Plasmid#178962PurposeExpresses human GFAP (R79C) mutant fused to 3xFLAG tag in mammalian cellsDepositorInsertGlial fibrillary acidic protein (GFAP Human)
UseTags3xFLAGExpressionMammalianMutationR79CPromoterCMV IE94 PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only