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Plasmid#138890PurposeEYFP reporter vector for the ZF43x2-Spaced-Far promoterDepositorInsertZF43x2-Spaced-Far
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x2-Spaced-FarAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD140 ZF43x12-Spaced-Mid_EYFP
Plasmid#138889PurposeEYFP reporter vector for the ZF43x12-Spaced-Mid promoterDepositorInsertZF43x12-Spaced-Mid
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x12-Spaced-MidAvailable sinceJan. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD138 ZF43x10-Spaced-Mid_EYFP
Plasmid#138888PurposeEYFP reporter vector for the ZF43x10-Spaced-Mid promoterDepositorInsertZF43x10-Spaced-Mid
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x10-Spaced-MidAvailable sinceJan. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD137 ZF43x8-Spaced-Mid_EYFP
Plasmid#138887PurposeEYFP reporter vector for the ZF43x8-Spaced-Mid promoterDepositorInsertZF43x8-Spaced-Mid
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x8-Spaced-MidAvailable sinceJan. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD136 ZF43x6-Spaced-Mid_EYFP
Plasmid#138886PurposeEYFP reporter vector for the ZF43x6-Spaced-Mid promoterDepositorInsertZF43x6-Spaced-Mid
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x6-Spaced-MidAvailable sinceJan. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD135 ZF43x4-Spaced-Mid_EYFP
Plasmid#138885PurposeEYFP reporter vector for the ZF43x4-Spaced-Mid promoterDepositorInsertZF43x4-Spaced-Mid
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x4-Spaced-MidAvailable sinceJan. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD134 ZF43x2-Spaced-Mid_EYFP
Plasmid#138884PurposeEYFP reporter vector for the ZF43x2-Spaced-Mid promoterDepositorInsertZF43x2-Spaced-Mid
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x2-Spaced-MidAvailable sinceJan. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lyn-YFP-Clover-FRB1N
Plasmid#162439Purposefor use in CIT (chemically induced trimerization) systemDepositorInsertLyn-YFP-Clover-FRB1N
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EBFP2-ccdB-EYFP
Plasmid#158558PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsGRK6[L66A E514A]-sYFP2
Plasmid#137781PurposeVisualization of GRK6DepositorInsertHuman GRK6[L66A E514A] (GRK6 Human)
UseTagsExpressionMammalianMutationL66A E514APromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV RhoB YFP MS2
Plasmid#131777PurposeRhoB upstream msfYFP 24xMS2 RhoB downstream for CRISPR taggingDepositorInsertmsfYFP - 24x MS2 loops
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV Btg2 YFP MS2
Plasmid#131776PurposeBtg2 upstream msfYFP 24xMS2 Btg2 downstream for CRISPR taggingDepositorInsertmsfYFP - 24x MS2 loops
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDM304_YFP-IqgC_mRFP-Raf1(RBD)
Plasmid#128698Purposesimultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cellsDepositorInsertsiqgC (full length)
Raf1 kinase (aa 50-132)
UseDictyostelium discoideum expression vectorTagsYFP and mRFPExpressionMutationnonePromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC601: pMVP (L3-L2) eYFP + polyA
Plasmid#121769PurposepMVP L3-L2 entry plasmid, contains eYFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest.DepositorInserteYFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LD001: pMVP (L3-L2) eYFP + WPRE
Plasmid#121777PurposepMVP L3-L2 entry plasmid, contains eYFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest in lentivirus vectors.DepositorInserteYFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO401: pMVP (R4-R3) eYFP open
Plasmid#121737PurposepMVP R4-R3 entry plasmid, contains eYFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteYFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_R89A-PolyA
Plasmid#112291PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Arginine 89 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_L91A-PolyA
Plasmid#112292PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Leucine 91 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_F95A-PolyA
Plasmid#112293PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Phenylalanine 95 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW sYFP2-T2A-mTurquoise2
Plasmid#118436PurposeProduces equimolar expression of sYFP2 and mTq2DepositorInsertsYFP2-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only