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Showing: 2401 - 2420 of 16061 results

  1. pREST-donor

    Plasmid
    #113807
    Purpose
    CRISPR donor plasmid to tag human transcription factor REST with GFP
    Depositor
    Kevin White
    Insert
    REST homology arms flanking EGFP-IRES-Neo cassette (REST Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs4864608
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113807-plasmid-map-sequence-id-231037

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  2. pcDNA3.1_FASN

    Plasmid
    #107138
    Purpose
    His tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells
    Depositor
    Brijesh Kumar Singh
    Insert
    Fatty acid synthase (FASN Human)
    Use
    Tags
    His
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    107138-plasmid-map-sequence-id-423159

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  9. eSrtA-MT4

    Plasmid
    #200306
    Purpose
    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    eSrtA-LPETG-MT4 (MT4 Human)
    Use
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    200306-plasmid-map-sequence-id-394863

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  10. eSrtA-MT3

    Plasmid
    #200305
    Purpose
    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    eSrtA-LPETG-MT3 (MT3 Human)
    Use
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    200305-plasmid-map-sequence-id-394866

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  14. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

    Plasmid
    #119864
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    HA-tagged biotin ligase (BirA), Thosea asigna vir…
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    119864-plasmid-map-sequence-id-239418

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  15. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Article
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Use
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

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  16. pPI463

    Plasmid
    #113217
    Purpose
    generic knockin vector for C-terminal tagging with mTurquoise2
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Generic knockin vector for c-terminal tagging wit…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113217-plasmid-map-sequence-id-217774

    Enlarge

  17. pPI193

    Plasmid
    #113224
    Purpose
    generic knockin vector for C-terminal tagging with mTurquoise2
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Generic knockin vector for c-terminal tagging wit…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113224-plasmid-map-sequence-id-217795

    Enlarge

Showing: 2401 - 2420 of 16061 results