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Plasmid#184976PurposeTest effect of extending a1/a2 on CAN1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationCAN1 donor G444stop, a1/a2 length extended to 27 …PromoterGal7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.111
Plasmid#184980PurposeTest effect of extending a1/a2 on TRP2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationTRP2 donor E64stop, a1/a2 length extended to 27 bpPromoterGal7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.113
Plasmid#184982PurposeTest effect of extending a1/a2 on FAA1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationFAA1 donor P233stop, a1/a2 length extended to 27 …PromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.039
Plasmid#184973PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 …PromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
puc19_N_tag
Plasmid#186650PurposeEmpty donor plasmid.DepositorInsertN-terminal 3xFlag-3xHA Tag
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-CfANLN
Plasmid#183837PurposeRepair template for the N-terminal tagging of anillin with mNeonGreen in canine cells using CRISPR/Cas9.DepositorInsertCanis familiaris ANLN homology arms with mNeonGreen-linker
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-whiteCopyCatcher
Plasmid#174062PurposeCopyCatcher insertion donor for the white locusDepositorInsertwhite (w Fly, Synthetic)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
LR (hsp70I: cPLA2-mK2)
Plasmid#162564PurposeMeasuring cPLA2 activation in live cells after heat shock.DepositorUseGateway donor vectorTagsmK2ExpressionMutationPromotercmlc2Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_HMG20A
Plasmid#86271PurposeDonor vector for 3' FLAG tag of human HMG20ADepositorInsertHMG20A homology arms (HMG20A Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_KLF11
Plasmid#86269PurposeDonor vector for 3' FLAG tag of human KLF11DepositorInsertKLF11 homology arms (KLF11 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCRISPR-HOT-P2A-Clover-BlastR
Plasmid#138568PurposeUniversal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJDepositorInsertP2A-clover-BlastR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCM-AAVS1-TO-hNGN2
Plasmid#105840PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuronDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
HR180-LGR5-GFP
Plasmid#129092PurposeKI EGFP with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertEGFP and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry
Plasmid#193465PurposeDn29 Donor with promoter for mcherry and puro expressionDepositorInsertDn29_attP
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only