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Plasmid#196500PurposeExpresses left-side TALE-DddAtox Cterm half Cox1-FusXTBE in mammalian cellsDepositorInsertCOX8A MTS-Cox1 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cox1-FusXTBE-Right TALE
Plasmid#196501PurposeExpresses Right-side TALE-DddAtox Nterm half Cox1-FusXTBE in mammalian cellsDepositorInsertCOX8A MTS-Cox1 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK033 p5E desma MCS
Plasmid#195948PurposeGateway p5E vectorDepositorInsertdesmin a regulatory elements (desma Zebrafish)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-TDP-43
Plasmid#162608PurposeAllows for transcription of TDP-43 mRNA for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP
Plasmid#164945PurposeTol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFPDepositorInsertKCNJ13 (KCNJ13 Human)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationStop codon deletedPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-jph1a
Plasmid#109497PurposeMuscle specific zebrafish expression of mKate2 fused to junctophilin 1aDepositorInsertjph1a (jph1a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPlease see Depositor CommentsPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTone-gata2aECE-nsGFP
Plasmid#132975Purposegata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backboneDepositorInsertsnuclear localized sfGFP
gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
UseUnspecified; Transposon-mediatedTags6x myc and SV40 NLSExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSi-check2-hRac2 - mut 3'UTR
Plasmid#97161PurposeFor dual luciferase report assayDepositorInsertRAC 3'UTR (AKT1 Human)
UseTagsExpressionMammalianMutationmiR-722 Binding MutantPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E_he1a:mCherry
Plasmid#113881Purposep3E entry vector for Three Multisite GatewayDepositorInserthe1a (he1.1 Zebrafish)
UseZebrafish danio expressionTagsmCherryExpressionMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E_he1a:eYFP
Plasmid#113879Purposep3E entry vector for Three Multisite GatewayDepositorInserthe1a (he1.1 Zebrafish)
UseZebrafish danio expressionTagseYFPExpressionMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E_he1a:ECFP
Plasmid#113880Purposep3E entry vector for Three Multisite GatewayDepositorInserthe1a (he1.1 Zebrafish)
UseZebrafish danio expressionTagsECFPExpressionMutationPromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
H2B CFP
Plasmid#198059PurposemCerulean fused to human H2B proteinDepositorInsertH2B
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmCeruleanExpressionMutationPromotersp6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUbi_dCas9-BFP-KRAB-gRNA
Plasmid#221503PurposeCRISPRi machinery fish codon optimized expressing plasmidDepositorInsertdCas9-tagBFP-KRAB
UseFish expressionTagsExpressionMutationD10A and H839A point mutationsPromoterZebrafish Ubiquitin promoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-PE2-His (IK1822)
Plasmid#170103PurposeT7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLSDepositorInsertbpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
UseCRISPRTags6xHis tagExpressionBacterialMutationBacteria codon-optimized nSpCas9 (H840A) & M-…PromoterT7Available sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
p13xQUAS-ZsGreen-P2A, α-cry:mCherry
Plasmid#180480PurposeInducible gene expression vector p13xQUAS-ZsGreen-P2A, α-cry:mCherryDepositorInsert13x QUAS-ZsGreen-P2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gfp-zf-vasa
Plasmid#167323PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.DepositorInsertzf vasa 3'UTRs (ddx4 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSGFPnERT(#205)
Plasmid#184065Purposecyclofen-inducible GFP nuclear relocation via mammalian cell transfection or mRNA synthesisDepositorInserteGFP-nls-ERT2
UseSynthetic BiologyTagsExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mCerulean 156-239-GGGS-TDP-43
Plasmid#162617PurposeAllows for transcription of mCerulean fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-I152L-GGGS-TDP-43
Plasmid#162614PurposeAllows for transcription of improved mVenus I152L fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT2-4xUAS:P2A-EGFP
Plasmid#127591PurposeThis vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with EGFP through P2A self-cleaving peptides.DepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only