Addgene

We narrowed to 5,112 results for: donor

Showing: 2381 - 2400 of 5112 results

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pFETCh_BCL6_iso1

Plasmid

#104038

Purpose

Donor vector for 3' FLAG tag of human BCL6_iso1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

BCL6_iso1 homology arms (BCL6 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-TRE-synPCB2.0

Plasmid

#139481

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Use

Tol2 transposon donor vector

Tags

Expression

Mammalian

Mutation

Promoter

Tet responsive element

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00120

Plasmid

#34274

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3956 (sna Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pORANGE Cacnb3-GFP KI

Plasmid

#139662

Purpose

Endogenous tagging of CaVβ3: C-terminal (amino acid position: W479)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_GATAD1

Plasmid

#104036

Purpose

Donor vector for 3' FLAG tag of human GATAD1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GATAD1 homology arms (GATAD1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mammalian

Mutation

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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KHBD00051

Plasmid

#34213

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10159 (BEAF-32 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pORANGE Cacnb2-GFP KI

Plasmid

#139661

Purpose

Endogenous tagging of CaVβ2: C-terminal (amino acid position: Q655)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00158

Plasmid

#34312

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG33183 (Hr46 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFETCh_KLF9

Plasmid

#86286

Purpose

Donor vector for 3' FLAG tag of human KLF9

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KLF9 homology arms (KLF9 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00203

Plasmid

#34354

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG17743 (pho Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00273

Plasmid

#34401

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11987 (tgo Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00343

Plasmid

#34466

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11992 (Rel Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00263

Plasmid

#34394

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1464 (ey Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00416

Plasmid

#34523

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG17950 (HmgD Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00327

Plasmid

#34450

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG18783 (Kr-h1 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
AAVS1-iPE2-Hygro

Plasmid

#214019

Purpose

inducible PE2 system for controllable prime editing; this plasmid is used to insert PE2 prime editor in one allele of AAVS1 locus

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

PE2

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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phROSA26-Tet-HA-JLP_V55Q

Plasmid

#208771

Purpose

Tetracycline inducible expression vector for HA-JLP_V55Q mutant protein, used as a donor plasmid for HA-JLP_V55Q knock-in at the human ROSA26 locus

Depositor

Katsuji Yoshioka

Article

Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

Insert

JLP (SPAG9 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG PSMA nanobody T-CAR

Plasmid

#171962

Purpose

Donor plasmid for PSMA nanobody T-CAR expression in human cells

Depositor

Xiaoping Bao

Article

Harris et al J Immunol Regen Med. 2023 May;20:100074.

Insert

Synthetic anti-PSMA nanobody CAR

Use

CRISPR, Synthetic Biology, and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSCL.040

Plasmid

#184974

Purpose

Test effect of extending a1/a2 on ADE2 editing rates in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2

Use

Tags

Expression

Yeast

Mutation

ADE2 donor P272stop, a1/a2 length extended to 27 …

Promoter

Gal7

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.109

Plasmid

#184978

Purpose

Test effect of extending a1/a2 on LYP1 editing rates in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1

Use

Tags

Expression

Yeast

Mutation

LYP1 donor E27stop, a1/a2 length extended to 27 bp

Promoter

Gal7

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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