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Plasmid#203228PurposeCo-expressing membrane-localized Voltron2 and membrane-localized CheRiff.DepositorInsertMembrane-localized optopatch for neuronal dendrites
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-NE2h-IRES-mCherry-CAAX
Plasmid#208691PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cellsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-NE2m-IRES-mCherry-CAAX
Plasmid#208690PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cellsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2m
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL1 CTF-FLAG
Plasmid#217706PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL1 (ADGRL1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-838PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
eGFP-CBP
Plasmid#169211PurposeExpression of eGFP-CBP protein for chitin visualizationDepositorInsertChitinase A1 Chitin Binding Domain
UseTags6xHis, Mxe Gyr Intein, and eGFPExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CAG_oROS-Gr
Plasmid#216113PurposeExpresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.DepositorInsertCAG_oROS-Gr
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL026_AAV_hSyn:SEP-GluA1
Plasmid#192519PurposeExpression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusionDepositorInsertSEP-GluA1
UseAAV and Cre/LoxTagssuper ecliptic phluorinExpressionMutationPromoterhSynAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PM-APEX2
Plasmid#215557PurposeStable, inducible expression in plasma membraneDepositorInsertLyn11-EGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1340-LV-DUAL-dGFP-NanoLuc-Puro
Plasmid#223160PurposeExpression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-lifeDepositorInsertdGFP-P2A-NanoLuc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-GOLGA2
Plasmid#207791PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393
Plasmid#216380PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTags3C-splitGFP1to10opt-ETVV and HA signal sequence-F…ExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggybac-EF1-H2AZ-halo
Plasmid#197907Purposesingle-particle tracking for H2AZDepositorInsertH2az2 (H2az2 Mouse)
UseTagsHalotagExpressionMammalianMutationPromoterEF1Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mScarlet-H2BC11
Plasmid#207756PurposeDonor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorArticleInsertH2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
lenti-GBA-N370S-nick sgRNA
Plasmid#214096PurposeLentiviral vector expressing nicking sgRNA to induce GBA N370S mutation using PE3DepositorInsertKRAS G13A nicking sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-GBA-N370S-tmpknot-epegRNA
Plasmid#214091PurposeLentiviral vector expressing epegRNA to induce GBA N370S mutationDepositorInsertGBA N370S epegRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
Plasmid#222991PurposeBacterial expression of iGeoCas9(C2) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA3 CTF-FLAG
Plasmid#217680PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRA3 (ADGRA3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-737PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA2 CTF-FLAG
Plasmid#217679PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRA2 (ADGRA2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-746PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPbU6_hdhfr/yfcu_Cas9
Plasmid#216423PurposeEmpty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RBD-JA1
Plasmid#167126PurposeExpresses SARS-CoV-2 RBD in bacterial systemDepositorInsertSARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
UseTagsExpressionBacterialMutationresidues (333 to 529 aa)PromoterT7Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only