Addgene

We narrowed to 3,246 results for: biorxiv

Showing: 2381 - 2400 of 3246 results

Results per page:

pTol2-elavl3-WHaloCaMP1a

Plasmid

#205312

Purpose

Expression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-WHaloCaMP1a-EGFP

Plasmid

#205313

Purpose

Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
p10xUAS-WHaloCaMP1a-EGFP

Plasmid

#205314

Purpose

Expression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driver

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn1-FLEX-CybSEP2-WPRE

Plasmid

#207661

Purpose

Monitoring neuropeptide release

Depositor

Sung Han

Article

Kim et al bioRxiv 2023.01.19.524797

Insert

Cytochrome b-561 (Cyb561 Mouse)

Use

AAV

Tags

Expression

Mutation

Histidine 86 and 159 to alanines, inserted 2 X SE…

Promoter

hSynapsin

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-iPE-Plus

Plasmid

#214062

Purpose

inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

PE-Plus

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-hP53DD (TET+CTD)

Plasmid

#214082

Purpose

Mammalian expression tetramerization domain (TET) and C-terminal negative regulatory domain (CTD) of human TP53

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

hP53DD (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-EGFR-L858R-T790M-dual-nick sgRNA

Plasmid

#214101

Purpose

Lentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.

Depositor

Ting Zhou

Article

Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.

Insert

EGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

Enlarge
pIR-CMV-SCN2A-Variant-4-IRES-mScarlet

Plasmid

#209410

Purpose

Expression of wild-type neonatal isoform of human SCN2A.

Depositor

Al George

Article

Thompson et al bioRxiv. 2023 Feb 23:2023.02.23.529757.

Insert

SCN2A Variant 4, stabilized (SCN2A Human)

Use

Tags

Expression

Mammalian

Mutation

IVS intron inserted after bp 4551 of the SCN2A OR…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

Plasmid

#216156

Purpose

Expresses mScarlet regardless of TDP-43 knockdown.

Depositor

Pietro Fratta

Article

Wilkins et al bioRxiv. 2023 Nov 15:2023.11.15.565967.

Insert

mScarlet with C-terminal FLAG tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-REGv2 Cryptic PE-Max

Plasmid

#216163

Purpose

Expression of prime editing vector PEMax (Plasmid #174820) but only in cells with TDP-43 loss of function

Depositor

Pietro Fratta

Article

Wilkins et al bioRxiv. 2023 Nov 15:2023.11.15.565967.

Insert

PEMax for prime editing with internal cryptic exon

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

Plasmid

#216164

Purpose

Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function

Depositor

Pietro Fratta

Article

Wilkins et al bioRxiv. 2023 Nov 15:2023.11.15.565967.

Insert

Gaussia princeps luciferase (with double mutation to improve stability)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-REGv1(mCherry-FLAG) in pTwist-CMV

Plasmid

#216165

Purpose

Expression of mCherry specifically in cells with TDP-43 knockdown. Uses AARS1-derived cryptic exon to control mCherry expression.

Depositor

Pietro Fratta

Article

Wilkins et al bioRxiv. 2023 Nov 15:2023.11.15.565967.

Insert

mCherry with C-terminal FLAG tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV2_CAG_oROS-HT(C199S)_WPRE

Plasmid

#216415

Purpose

Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT(C199S)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pGlomyc-Klf4

Plasmid

#172870

Purpose

Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Klf4 (Klf4 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT360 AAV-Rapamycin Inducible-noActivator Domain -Citrine

Plasmid

#202058

Purpose

Inducible AAV with reporter gene and cloning site to add activator domains

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al bioRxiv. (PrePrint)

Insert

AAV-Rapamycin Inducible-noActivator Domain -Citrine

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITRs intact

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT312 AAV-Rapamycin Inducible-NFZ-Citrine with sNRP-1 terminator

Plasmid

#202061

Purpose

Inducible AAV activator

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al bioRxiv. (PrePrint)

Insert

AAV-Rapamycin Inducible-NFZ-Citrine with sNRP-1 terminator

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITRs intact

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

Plasmid

#194279

Purpose

Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

Depositor

Charles Gersbach

Article

McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

Inserts

humanized VP64 dSaCas9 VP64 T2A Thy1.1
gRNA

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

hU6 and hUbC

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-060

Plasmid

#197133

Purpose

CRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expression

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Type

Empty backbone

Use

Lentiviral

Tags

EGFP and mCherry

Expression

Mammalian

Mutation

MCP(N55K)

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-117

Plasmid

#197134

Purpose

CloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-119

Plasmid

#197135

Purpose

CloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge