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Plasmid#131138PurposeUASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorInsertCrk (Crk Fly)
UseTags3XFLAG and tdTomatoExpressionInsectMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_6E
Plasmid#98655PurposeExpresses MBP-tagged full length phosphomimetic FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation6 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-3xVenusYFP-OcsT
Plasmid#71271PurposeEntry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsert3 times VenusYFP
UseGatewayTagsoctaline synthase terminatorExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTIGER_mNeonGreen::3xFLAG::dCrk
Plasmid#131137PurposeUASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorInsertCrk (Crk Fly)
UseTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-GUS-OcsT
Plasmid#71267PurposeEntry clone containing the GUS enzyme. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertBeta-glucuronidase
UseGatewayTags2xGly linker and octaline synthase terminatorExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc-oDam_f_GFPoNLS
Plasmid#85818PurposeiDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc oDam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ATG13 (363-517)-ULK1 (836-1050)-TSF
Plasmid#203549PurposeMammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complexDepositorUseTagsTwinStrep FlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TSF-ATG13 (450-517)-ULK1 (836-1050)
Plasmid#203548PurposeMammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complexDepositorUseTagsTwinStrep FlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)
Plasmid#203547PurposeMammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complexDepositorUseTagsTwinStrep FlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1149-1376_AC
Plasmid#148475PurposeInsect Expression of DmNot1_1149-1376DepositorInsertDmNot1_1149-1376 (Not1 Fly)
UseTagsExpressionInsectMutation1 silent mutation compared to the sequence given …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-Q294AN296F-dsRNAres_W
Plasmid#147862PurposeInsect Expression of DmMe31B-Q294AAN296F-dsRNAresDepositorInsertDmMe31B-Q294AAN296F-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-Q294AN296FR347A-dsRNAres_W
Plasmid#147863PurposeInsect Expression of DmMe31B-Q294AAN296FR347A-dsRNAresDepositorInsertDmMe31B-Q294AAN296FR347A-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-R347A-dsRNAres_W
Plasmid#147864PurposeInsect Expression of DmMe31B-R347A-dsRNAresDepositorInsertDmMe31B-R347A-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-S291A-dsRNAres_W
Plasmid#147865PurposeInsect Expression of DmMe31B-S291A-dsRNAresDepositorInsertDmMe31B-S291A-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182_635-1384_W
Plasmid#147831PurposeInsect Expression of DmGW182_635-1384DepositorInsertDmGW182_635-1384 (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso2-E2302AI2313R_U
Plasmid#147734PurposeMammalian Expression of HsNot1iso2-E2302AI2313RDepositorInsertHsNot1iso2-E2302AI2313R (CNOT1 Human)
UseTagsExpressionMammalianMutation4 silent and two non silent G917E and R2353Q muta…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso2-R2097DI2313R_U
Plasmid#147735PurposeMammalian Expression of HsNot1iso2-R2097DI2313RDepositorInsertHsNot1iso2-R2097DI2313R (CNOT1 Human)
UseTagsExpressionMammalianMutation4 silent and two non silent G917E and R2353Q muta…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso2-3xmut_U
Plasmid#147731PurposeMammalian Expression of HsNot1iso2-3xmutDepositorInsertHsNot1iso2-3xmut (CNOT1 Human)
UseTagsExpressionMammalianMutation4 silent and two non silent G917E and R2353Q muta…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso2-8xmut_U
Plasmid#147733PurposeMammalian Expression of HsNot1iso2-8xmutDepositorInsertHsNot1iso2-8xmut (CNOT1 Human)
UseTagsExpressionMammalianMutation4 silent and two non silent G917E and R2353Q muta…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-del1967-2251-dsRNAres_U
Plasmid#147711PurposeInsect Expression of DmNot1-del1967-2251-dsRNAresDepositorInsertDmNot1-del1967-2251-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only