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Bacterial Strain#215356PurposeVibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.DepositorBacterial ResistanceNoneAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only
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V. natriegens NC1
Bacterial Strain#215355PurposeVibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.DepositorBacterial ResistanceNoneAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
CSH130+pRCP
Bacterial Strain#195860PurposeWT E. coli strain transformed with plasmid carrying WT chromogenic protein (CP). Serves as control for suppressor strains. Grows purple when CP is induced with rhamnose.DepositorBacterial ResistanceChloramphenicolSpeciesAcropora milleporaAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
CSH130+pRCPam
Bacterial Strain#195859PurposeWT E. coli strain transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Serves as control for suppressor strains. No color when grown with or without rhamnose.DepositorBacterial ResistanceChloramphenicolSpeciesAcropora milleporaAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
RP4-8
Plasmid#79813PurposeModified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugationDepositorInsertGentamicin resistance gene
UseHelper bhr plasmid for mobilizing other plasmids …TagsExpressionMutationPromoterOwn promoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
RP4-7
Plasmid#79811PurposeModified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmidDepositorInsertChloramphenicol acetyltransferase
UseHelper plasmid for mobilizationTagsExpressionMutationPromoterOwn promoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
RP4-11
Plasmid#79812PurposeModified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmidDepositorInsertKanamycin cassette and SacB gene (sacB )
UseHelper plasmid for mobilizationTagsExpressionMutationPromoterKmAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
BPK1520_blastR
Plasmid#175289PurposeHuman expression plasmid for SpCas9 sgRNA with blasticidin co-selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCMV/U6Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
p11-3’_8xN_PAM-site1 (RTW554)
Pooled Library#160132PurposePlasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)DepositorSpeciesSyntheticUseCRISPRAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p11-5’_10xN_PAM-site4 (RTW574)
Pooled Library#160135PurposePlasmid library harboring a randomized 10nt PAM 5’ of target site 4. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)DepositorSpeciesSyntheticUseCRISPRAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p11-3’_8xN_PAM-site2 (RTW555)
Pooled Library#160133PurposePlasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)DepositorSpeciesSyntheticUseCRISPRAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-nEF Con/Fon DREADD Gq-mCherry
Plasmid#183532PurposeINTRSECT/ChemogeneticsDepositorInsertDREADD Gq-mCherry
UseAAVTagsExpressionMutationNonePromoternEFAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF Coff/Fon DREADD Gq-mCherry
Plasmid#183534PurposeINTRSECT/ChemogeneticsDepositorInsertDREADD Gq-mCherry
UseAAVTagsExpressionMutationNonePromoternEFAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF Con/Foff DREADD Gq-mCherry
Plasmid#183533PurposeINTRSECT/ChemogeneticsDepositorInsertDREADD Gq-mCherry
UseAAVTagsExpressionMutationNonePromoternEFAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecBCD
Bacterial Strain#176583PurposeE. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.DepositorBacterial ResistanceChloramphenicolAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecB
Bacterial Strain#176582PurposeE. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.DepositorBacterial ResistanceChloramphenicolAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAdDeltaF6
Plasmid#112867PurposeAAV helper plasmidDepositorInsertE4, E2a and VA
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
dSaCas9-KRAB
Plasmid#205250PurposeIn vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.DepositorInsertdSaCas9-KRAB
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-iCre
Plasmid#122518PurposeCre expressionDepositorInsertiCre
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only